Mouse Picalm activation kit by CRISPRa

SKU GA214457

Picalm CRISPRa kit - CRISPR gene activation of mouse phosphatidylinositol binding clathrin assembly protein

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Picalm
• Locus ID: 233489
• Components:

  GA214457G1, Picalm gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA214457G2, Picalm gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA214457G3, Picalm gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001252520, NM_001252521, NM_001252522, NM_001252523, NM_001252524, NM_146194, NM_001360867
• UniProt ID: Q7M6Y3
• Synonyms: CALM; CLTH; fit-1; fit1; mKIAA4114; PAP180
• Summary: Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).[UniProtKB/Swiss-Prot Function]