Mouse Mib1 activation kit by CRISPRa

SKU GA213756

Mib1 CRISPRa kit - CRISPR gene activation of mouse mindbomb E3 ubiquitin protein ligase 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Mib1
• Locus ID: 225164
• Components:

  GA213756G1, Mib1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA213756G2, Mib1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA213756G3, Mib1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144860, NM_001364997
• UniProt ID: Q80SY4
• Synonyms: DIP-1; E430019M12Rik; Mib; mKIAA1323
• Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation (By similarity). Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.[UniProtKB/Swiss-Prot Function]