Mouse Pja2 activation kit by CRISPRa

SKU GA213744

Pja2 CRISPRa kit - CRISPR gene activation of mouse praja ring finger ubiquitin ligase 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Pja2
• Locus ID: 224938
• Components:

  GA213744G1, Pja2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA213744G2, Pja2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA213744G3, Pja2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001025309, NM_144859
• UniProt ID: Q80U04
• Synonyms: AI447901; AL022700; mKIAA0438; Neurodap1
• Summary: Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype.[UniProtKB/Swiss-Prot Function]