Mouse Gtf2h3 activation kit by CRISPRa

SKU GA212928

Gtf2h3 CRISPRa kit - CRISPR gene activation of mouse general transcription factor IIH, polypeptide 3

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Gtf2h3
• Locus ID: 209357
• Components:

  GA212928G1, Gtf2h3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA212928G2, Gtf2h3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA212928G3, Gtf2h3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181410
• UniProt ID: Q8VD76
• Synonyms: 34kDa; 5033417D07Rik; BTF2; BTF2 p34; C730029A10; D5Ertd679e; TFIIH
• Summary: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]