Mouse Acot4 activation kit by CRISPRa

SKU GA212662

Acot4 CRISPRa kit - CRISPR gene activation of mouse acyl-CoA thioesterase 4

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Acot4
• Locus ID: 171282
• Components:

  GA212662G1, Acot4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA212662G2, Acot4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA212662G3, Acot4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134247
• UniProt ID: Q8BWN8
• Synonyms: B430212I04Rik; PTE-Ib; Pte1b; Pte2b
• Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16141203, PubMed:16940157). In contrast to human peroxisomal succinyl-coenzyme A thioesterase/ACOT4, mouse ACOT4 is essentially a succinyl-CoA thioesterase with no activity with medium to long chain saturated acyl-CoAs and with a low activity toward glutaryl-CoA (PubMed:16141203, PubMed:16940157).[UniProtKB/Swiss-Prot Function]