Mouse Phactr4 activation kit by CRISPRa

SKU GA211587

Phactr4 CRISPRa kit - CRISPR gene activation of mouse phosphatase and actin regulator 4

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Phactr4
• Locus ID: 100169
• Components:

  GA211587G1, Phactr4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA211587G2, Phactr4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA211587G3, Phactr4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001161797, NM_175306
• UniProt ID: Q501J7
• Synonyms: 3110001B12Rik; AI527228; AW495572; C330013F19Rik; mKIAA4120; N28169
• Summary: Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway.[UniProtKB/Swiss-Prot Function]