Mouse Rictor activation kit by CRISPRa

SKU GA211024

Rictor CRISPRa kit - CRISPR gene activation of mouse RPTOR independent companion of MTOR, complex 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Rictor
• Locus ID: 78757
• Components:

  GA211024G1, Rictor gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA211024G2, Rictor gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA211024G3, Rictor gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030168
• UniProt ID: Q6QI06
• Synonyms: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik
• Summary: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.[UniProtKB/Swiss-Prot Function]