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Mouse Top1mt activation kit by CRISPRa

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SKU
GA209376
Top1mt CRISPRa kit - CRISPR gene activation of mouse DNA topoisomerase 1, mitochondrial
  $1,657.00
4 Weeks*

Other products for "Top1mt"

Specifications
Specifications
Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Target Symbol Top1mt
Locus ID 72960
Components

GA209376G1, Top1mt gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA209376G2, Top1mt gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA209376G3, Top1mt gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
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Reference Data
RefSeq NM_028404, NM_001355589
UniProt ID Q8R4U6
Synonyms 2900052H09Rik
Summary Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
KN518058 Top1mt - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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