Mouse Pitrm1 activation kit by CRISPRa

SKU GA208248

Pitrm1 CRISPRa kit - CRISPR gene activation of mouse pitrilysin metallepetidase 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Pitrm1
• Locus ID: 69617
• Components:

  GA208248G1, Pitrm1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA208248G2, Pitrm1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA208248G3, Pitrm1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145131, NM_001360106
• UniProt ID: Q8K411
• Synonyms: 2310012C15Rik; AA410010; mKIAA1104; MP-1; MP1; Ntup1; PreP
• Summary: Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently.[UniProtKB/Swiss-Prot Function]