Mouse Ncbp3 activation kit by CRISPRa

SKU GA207003

Ncbp3 CRISPRa kit - CRISPR gene activation of mouse nuclear cap binding subunit 3

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Ncbp3
• Locus ID: 66874
• Components:

  GA207003G1, Ncbp3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA207003G2, Ncbp3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA207003G3, Ncbp3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025818
• UniProt ID: Q8BZR9
• Synonyms: 1200014J11Rik; C78393; C130061O14Rik
• Summary: Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).[UniProtKB/Swiss-Prot Function]