Mouse Nampt activation kit by CRISPRa

SKU GA206340

Nampt CRISPRa kit - CRISPR gene activation of mouse nicotinamide phosphoribosyltransferase

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Nampt
• Locus ID: 59027
• Components:

  GA206340G1, Nampt gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA206340G2, Nampt gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA206340G3, Nampt gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021524
• UniProt ID: Q99KQ4
• Synonyms: 1110035O14Rik; AI314458; AI480535; NAmPRTase; Pbef; Pbef1; Visfatin
• Summary: The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.[UniProtKB/Swiss-Prot Function]