Mouse Rnf138 activation kit by CRISPRa

SKU GA206013

Rnf138 CRISPRa kit - CRISPR gene activation of mouse ring finger protein 138

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Rnf138
• Locus ID: 56515
• Components:

  GA206013G1, Rnf138 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA206013G2, Rnf138 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA206013G3, Rnf138 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001303005, NM_001303011, NM_019706, NM_207623
• UniProt ID: Q9CQE0
• Synonyms: 2410015A17Rik; 2810480D20Rik; STRIN; Trif; Trif-d
• Summary: E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination. Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination. Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination. According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.[UniProtKB/Swiss-Prot Function]