Mouse Tyro3 activation kit by CRISPRa

SKU GA204506

Tyro3 CRISPRa kit - CRISPR gene activation of mouse TYRO3 protein tyrosine kinase 3

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Tyro3
• Locus ID: 22174
• Components:

  GA204506G1, Tyro3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA204506G2, Tyro3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA204506G3, Tyro3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001290800, NM_019392
• UniProt ID: P55144
• Synonyms: AI323366; Brt; Dtk; Etk-2; etk2/tyro3; Rse; Sky; Tif; tk19-1; TK19-2
• Summary: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function]