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Mouse Top3b activation kit by CRISPRa

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SKU
GA204416
Top3b CRISPRa kit - CRISPR gene activation of mouse topoisomerase (DNA) III beta
  $1,657.00
4 Weeks*

Other products for "Top3b"

Specifications
Specifications
Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Target Symbol Top3b
Locus ID 21976
Components

GA204416G1, Top3b gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA204416G2, Top3b gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA204416G3, Top3b gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
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Reference Data
RefSeq NM_001326576, NM_011624
UniProt ID Q9Z321
Summary Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
KN518062 Top3b - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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