Mouse Mre11a activation kit by CRISPRa

SKU GA202713

Mre11a CRISPRa kit - CRISPR gene activation of mouse MRE11A homolog A, double strand break repair nuclease

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Mre11a
• Locus ID: 17535
• Components:

  GA202713G1, Mre11a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA202713G2, Mre11a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA202713G3, Mre11a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001310728, NM_018736
• UniProt ID: Q61216
• Synonyms: Mre11; Mre11b
• Summary: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.[UniProtKB/Swiss-Prot Function]