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Mouse Cyp1b1 activation kit by CRISPRa

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SKU
GA200972
Cyp1b1 CRISPRa kit - CRISPR gene activation of mouse cytochrome P450, family 1, subfamily b, polypeptide 1
  $1,657.00
4 Weeks*

Other products for "Cyp1b1"

Specifications
Specifications
Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Target Symbol Cyp1b1
Locus ID 13078
Components

GA200972G1, Cyp1b1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA200972G2, Cyp1b1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA200972G3, Cyp1b1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
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Reference Data
RefSeq NM_009994, NM_001364889
UniProt ID Q64429
Synonyms CP1B; P4501b1
Summary A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (By similarity). Exhibits catalytic activity for the formation of hydroxyestrogens from 17beta-estradiol (E2), namely 2- and 4-hydroxy E2 (PubMed:23821647). Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (By similarity). May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:15258110). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed:15258110). Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (By similarity). Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (By similarity). Plays an important role in retinal vascular development. Under ambient/hyperoxic O2 conditions, promotes angiogenesis and capillary morphogenesis of retinal endothelial cells and pericytes, likely by metabolizing the oxygenated products symptomatic of oxidative stress (PubMed:19005183, PubMed:20032512, PubMed:23568032). Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (PubMed:23979599).UniProtKB/Swiss-Prot Function
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Documents
Resources
Other Versions
SKU Description Size Price
KN504095 Cyp1b1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

The loss of microglia activities facilitates glaucoma progression in association with CYP1B1 gene mutation (p.Gly61Glu)
Alghamdi, Aldossary, Albahkali et al
PLoS One (2020) 15 (11), e0241902 DOI: 10.1371/journal.pone.0241902
Cited by 7
AI Summary: OriGene’s custom-designed CRISPR pCas-Guide kit for CYP1B1 (c.182G>A, p.Gly61Glu) was used to introduce the specific gene mutation into target cells, enabling the study of its effects on microglia, astrocytes, and mesenchymal stem cells in glaucoma progression.
Snippet: CRISPR editing of target cells. A custom-designed CRISPR kit (Origene, Rockville, MD, USA) for CYP1B1 (c.182G>A, p.Gly61Glu, a CRISPR pCas-Guide) was used, as previously described in other studies [22, 23]. In the pCas-Guide, the following modified sequence Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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