Mouse Akap1 activation kit by CRISPRa

SKU GA200143

Akap1 CRISPRa kit - CRISPR gene activation of mouse A kinase (PRKA) anchor protein 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Akap1
• Locus ID: 11640
• Components:

  GA200143G1, Akap1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA200143G2, Akap1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA200143G3, Akap1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042541, NM_009648, NR_104310
• UniProt ID: O08715
• Synonyms: Akap; AKAP84; AKAP121; C76494; C81186; DAKAP1; S-AKAP84
• Summary: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.[UniProtKB/Swiss-Prot Function]