Human KBTBD6 activation kit by CRISPRa

SKU GA113956

KBTBD6 CRISPRa kit - CRISPR gene activation of human kelch repeat and BTB domain containing 6

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: KBTBD6
• Locus ID: 89890
• Components:

  GA113956G1, KBTBD6 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA113956G2, KBTBD6 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA113956G3, KBTBD6 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152903
• UniProt ID: Q86V97
• Synonyms: DKFZp547E1912; DKFZp547M073; FLJ58260
• Summary: As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation (PubMed:25684205). By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed:25684205). Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex (PubMed:25684205).[UniProtKB/Swiss-Prot Function]