Human B7H3 (CD276) activation kit by CRISPRa

SKU GA112946

CD276 CRISPRa kit - CRISPR gene activation of human CD276 molecule

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: CD276
• Locus ID: 80381
• Components:

  GA112946G1, B7H3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA112946G2, B7H3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA112946G3, B7H3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001024736, NM_025240, NM_001329628, NM_001329629
• UniProt ID: Q5ZPR3
• Synonyms: 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
• Summary: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]