Human TASOR activation kit by CRISPRa

SKU GA108181

TASOR CRISPRa kit - CRISPR gene activation of human transcription activation suppressor

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: TASOR
• Locus ID: 23272
• Components:

  GA108181G1, TASOR gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA108181G2, TASOR gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA108181G3, TASOR gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001112736, NM_015224, NM_001365635, NM_001365636, NM_001363940, NM_001365637, NM_001365638
• UniProt ID: Q9UK61
• Synonyms: C3orf63; RAP140; se89-1
• Summary: Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression (PubMed:26022416, PubMed:28581500). The HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).[UniProtKB/Swiss-Prot Function]