Human MED14 activation kit by CRISPRa

SKU GA106190

MED14 CRISPRa kit - CRISPR gene activation of human mediator complex subunit 14

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: MED14
• Locus ID: 9282
• Components:

  GA106190G1, MED14 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA106190G2, MED14 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA106190G3, MED14 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004229
• UniProt ID: O60244
• Synonyms: CRSP2; CRSP150; CSRP; CXorf4; DRIP150; EXLM1; RGR1; TRAP170
• Summary: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]