Human Mel18 (PCGF2) activation kit by CRISPRa

SKU GA105262

PCGF2 CRISPRa kit - CRISPR gene activation of human polycomb group ring finger 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: PCGF2
• Locus ID: 7703
• Components:

  GA105262G1, Mel18 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105262G2, Mel18 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105262G3, Mel18 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007144, NM_001369614, NM_001369615
• UniProt ID: P35227
• Synonyms: MEL-18; RNF110; ZNF144
• Summary: The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]