Human PKM2 (PKM) activation kit by CRISPRa

SKU GA103576

PKM CRISPRa kit - CRISPR gene activation of human pyruvate kinase M1/2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: PKM
• Locus ID: 5315
• Components:

  GA103576G1, PKM2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103576G2, PKM2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103576G3, PKM2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001206796, NM_001206797, NM_001206798, NM_001206799, NM_001316318, NM_002654, NM_182470, NM_182471
• UniProt ID: P14618
• Synonyms: CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1
• Summary: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]