Human MCM2 activation kit by CRISPRa

SKU GA102844

MCM2 CRISPRa kit - CRISPR gene activation of human minichromosome maintenance complex component 2

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: MCM2
• Locus ID: 4171
• Components:

  GA102844G1, MCM2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102844G2, MCM2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102844G3, MCM2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004526, NR_073375
• UniProt ID: P49736
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN
• Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]