Human MRPL49 activation kit by CRISPRa

SKU GA100515

MRPL49 CRISPRa kit - CRISPR gene activation of human mitochondrial ribosomal protein L49

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: MRPL49
• Locus ID: 740
• Components:

  GA100515G1, MRPL49 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100515G2, MRPL49 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100515G3, MRPL49 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004927, NR_037567, NR_037568
• UniProt ID: Q13405
• Synonyms: C11orf4; L49mt; MRP-L49; NOF; NOF1
• Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011]