Human B Raf (BRAF) activation kit by CRISPRa

SKU GA100469

BRAF CRISPRa kit - CRISPR gene activation of human B-Raf proto-oncogene, serine/threonine kinase

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: BRAF
• Locus ID: 673
• Components:

  GA100469G1, B Raf gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100469G2, B Raf gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100469G3, B Raf gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004333, NM_001354609, NR_148928
• UniProt ID: P15056
• Synonyms: B-raf; B-RAF1; BRAF1; NS7; RAFB1
• Summary: This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]