Human APAF1 activation kit by CRISPRa

SKU GA100218

APAF1 CRISPRa kit - CRISPR gene activation of human apoptotic peptidase activating factor 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: APAF1
• Locus ID: 317
• Components:

  GA100218G1, APAF1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100218G2, APAF1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100218G3, APAF1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001160, NM_013229, NM_181861, NM_181868, NM_181869
• UniProt ID: O14727
• Synonyms: APAF-1; CED4
• Summary: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]