OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

AAVS1-int5 PCR primer pairs

Be the first to review this product
SKU
GE100033
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
  $142.00
In Stock*

Frequently Bought Together (4)

TA811179 CAS9 mouse monoclonal antibody,clone OTI5E5 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
500 pmol
$150.00
TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
500 pmol
$160.00
Specifications
Specifications
Product Data
Amplicon Size 1575 bp
PCR Conditions
Step Temperature Time
1 98°C 30s
2 98°C 10s
3 60°C 30s
4 72°C 1min
Step 2-4 X 34
6 72°C 5min
7 4°C hold
Reconstitution Method 2 nmol lyophilized. Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended primers are stable for one year from date of shipping when stored at -20°C.
Shipping Ambient
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Omarigliptin alleviates cognitive dysfunction in Streptozotocin-induced diabetic mouse
Li, Yin, Li et al
Bioengineered (2022) 13 (4), 9387-9396 DOI: 10.1080/21655979.2022.2055699
Cited by 16
AI Summary: OriGene contributed to this study by providing PCR primers for SIRT3 and GAPDH, which were used to measure gene expression levels related to Omarigliptin's effects on cognitive dysfunction in diabetic mice.
Snippet: (Bio-Rad Laboratories, Hercules, CA, USA). The mRNA level of SIRT3 was calculated by the ΔΔCT-method relative to GAPDH [22]. PCR primers used in this study were purchased from a commercial source (Origene, USA). The sequences of primers include: SIRT-3 Upstream 5’-GCTACATGCACGGTCTGTCGAA-3’, SIRT-3 Downstream: 5’-CAATGTCGGGTTTCACAACGCC-3’;GAPDH Upstream 5’-CATCACTGCCACCCAGAAGACTG-3’;- PCR primers...
Long non‑coding RNA nuclear paraspeckle assembly transcript 1 regulates ionizing radiation‑induced pyroptosis via microRNA‑448/gasdermin E in colorectal cancer cells
Su, Duan, Zhu et al
Int J Oncol (2021) 59 (4) DOI: 10.3892/ijo.2021.5259
Cited by 48
AI Summary: OriGene Technologies Inc. contributed PCR primers used for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analyses in this study.
Snippet: Laboratories, Inc.). PCR primer sequences were obtained from OriGene Technologies Inc. or designed using NCBI Primer-BLAST (https://www.ncbi.nlm.nih.gov/ tools/primer-blast/). Primer sequences were listed in Table SI. The
FigureFigureFigure
Chronic Inflammation in Ulcerative Colitis Causes Long-Term Changes in Goblet Cell Function
Singh, Johnson, Yin et al
Cell Mol Gastroenterol Hepatol (2021) 13 (1), 219-232 DOI: 10.1016/j.jcmgh.2021.08.010
Cited by 87
AI Summary: OriGene Technologies contributed to the study by providing commercially available primer pairs (LGR5: HP207145; Ki67: HP206104; ATOH1: HP208359; NEUROG3: HP213982; SPDEF: HP210328; MUC2: HP206138; CHGA: HP20519) used for quantitative real-time PCR analysis of gene expression in colonoids.
Snippet: …The following primer pairs were used: LGR5: HP207145;Ki67: HP206104;ATOH1: HP208359;NEUROG3: HP213982;SPDEF: HP210328;MUC2: HP206138;CHGA: HP20519;Commercially available primer pairs from OriGene Technologies (Rockville, MD) were used
A naturally occurring polymorphism in the base of Sudan virus glycoprotein decreases glycoprotein stability in a species-dependent manner
Lennemann, Dillard, Ruggio et al
J Virol (2021)
AI Summary: OriGene contributed to this study by providing the NPC1 ORF plasmid, which was used to generate a CT43-NPC1-myc stable cell line for investigating Sudan virus glycoprotein entry mechanisms.
Snippet: 476 Generation of CT43-NPC1-myc stable cell line. The NPC1 ORF was PCR amplified from a 477 plasmid obtained from OriGene (Rockville, MD) and inserted upstream of a c-myc tag (amino 478 acids: EQKLISEEDL) in pcDNA3.1 (Invitrogen)
Soluble (pro)renin receptor promotes fibrotic response in renal proximal tubule epithelial cells in vitro via Akt/β-catenin/Snail signaling pathway
Xie, Su, Lu et al
Am. J. Physiol. Renal Physiol. (2020)
Cited by 33
AI Summary: OriGene contributed qPCR primer pairs (Gene Expression qPCR Primer Pairs) used for gene expression analysis in this study, enabling accurate measurement of target gene expression levels related to fibrotic responses in HK-2 cells.
Snippet: (Applied Biosystems). GAPDH was detected as an internal control. Primer sequences were 120 designed by OriGene Technologies (available at 121 https://www.origene.com/category/ gene-expression/qpcr-primer-pairs). 122 Immunofluorescence Analysis 123
Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling
Zhang, Liu, Zong
Exp Ther Med (2019) 18 (6), 4781-4789 DOI: 10.3892/etm.2019.8127
AI Summary: In this study, OriGene products—specifically anti-mouse (cat. no. 127655) and anti-rabbit (cat. no. 125946) antibodies, as well as D-glucose—were used for protein detection and cell culture, supporting the investigation of triptolide’s protective effects against TGF-β1-induced pulmonary fibrosis.
Snippet: (cat. nos. anti‑mouse 127655 and anti‑rabbit 125946) and D‑glucose were purchased from OriGene Technologies, Inc. PCR primers were obtained from Western Biotech. Co., Ltd. Calpeptin (calpain inhibitor) and FAK inhibitor were purchased from Sigma‑Aldrich
FigureFigureFigure
Showing 1-6 of 48 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products