M1 (NC_007377) Virus Tagged ORF Clone

  • TrueORF®
SKU
VC102451
Myc-DDK-tagged ORF clone of viral ORF for membrane protein M1 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308854
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol M1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102451 represents NCBI reference of YP_308854 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCTCCTGACCGAAGTCGAGACATATGTGCTGTCTATTGTCCCGAGTGGCCCTCTGAAGGCGGAGA
TCGCCCAACGACTGGAGGATGTTTTTGCGGGGAAAAACACTGACCTCGAGGCCCTGATGGAATGGCTGAA
GACCCGGCCGATTTTGAGTCCCCTTACAAAGGGTATACTTGGATTTGTATTCACCCTGACAGTGCCTAGT
GAGAGAGGCTTGCAGAGGAGACGCTTCGTCCAGAATGCCCTGAATGGAAACGGAGACCCTAATAACATGG
ACAGAGCCGTGAAGCTTTATCGCAAACTGAAGAGGGAGATTACCTTCCACGGCGCCAAGGAGGTTGCCCT
CTCCTATTCAGCGGGAGCTCTCGCAAGCTGCATGGGGCTGATTTATAACCGAATGGGCGCGGTCACAACG
GAGGTGGCTTTTGCGGTCGTGTGTGCCACTTGCGAGCAGATTGCCGATAGCCAGCACCGCAGCCACCGAC
AGATGGTCACTACAACCAATCCTCTCATCAGACATGAAAACCGGATGGTCCTTGCATCTACCACCGCTAA
GGCGATGGAGCAGATGGCCGGTAGCTCCGAGCAGGCTGCTGAAGCCATGGAAGTTGCGAGTCAAGCACGG
CAGATGGTGCAGGCGATGCGGGCGATTGGAACTCCTCCAAGCAGCAGCGCCGGCCTTAAAGACGACCTTC
TCGAGAATCTGCAGGCGTACCAGAAAAGGATGGGGGTGCAGATGCAGAGATTCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>VC102451 representing YP_308854
Red=Cloning sites Green=Tags

MSLLTEVETYVLSIVPSGPLKAEIAQRLEDVFAGKNTDLEALMEWLKTRPILSPLTKGILGFVFTLTVPS
ERGLQRRRFVQNALNGNGDPNNMDRAVKLYRKLKREITFHGAKEVALSYSAGALASCMGLIYNRMGAVTT
EVAFAVVCATCEQIADSQHRSHRQMVTTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAAEAMEVASQAR
QMVQAMRAIGTPPSSSAGLKDDLLENLQAYQKRMGVQMQRFK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_007377
ORF Size 756 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_007377.1, YP_308854
RefSeq ORF 756 bp
Locus ID 3655108
MW 27.8 kDa
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Documents
Resources
"NC_007377" in other vectors (1)
SKU Description Size Price
VC102450 Myc-DDK-tagged ORF clone of viral ORF for membrane protein M2 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308853 10 ug
$165.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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