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M2 (NC_007377) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102450
Myc-DDK-tagged ORF clone of viral ORF for membrane protein M2 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308853
  $165.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol M2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102450 represents NCBI reference of YP_308853 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCTGCTCACTGAAGTCGAGACGCCAATACGGAACGAATGGGGATGCAGGTGCAATGACTCCAGCG
ATCCTCTGGTGGTGGCCGCTTCTATTATTGGCATTCTGCACTTCATTCTTTGGATCTTGGACAGACTCTT
CTTTAAATGTATATATCGCTTTTTTAAACACGGATTGAAGCGCGGTCCATCAACCGAGGGTGTGCCAGAG
AGCATGAGGGAGGAGTACCGCAAGGAGCAGCAAAGCGCCGTAGATGCCGATGACTCCCATTTTGTGTCAA
TCGAGTTGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102450 representing YP_308853
Red=Cloning sites Green=Tags

MSLLTEVETPIRNEWGCRCNDSSDPLVVAASIIGILHFILWILDRLFFKCIYRFFKHGLKRGPSTEGVPE
SMREEYRKEQQSAVDADDSHFVSIELE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_007377
ORF Size 291 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_007377.1, YP_308853
RefSeq ORF 291 bp
Locus ID 3655107
MW 11.2 kDa
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"NC_007377" in other vectors (1)
SKU Description Size Price
VC102451 Myc-DDK-tagged ORF clone of viral ORF for membrane protein M1 [Influenza A virus (A/Korea/426/68(H2N2))], codon optimized for human cell expression, YP_308854 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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