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L1 (NC_001355) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC102000
Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040304
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC102000 represents NCBI reference of NP_040304 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCGGCCGTCCGATAGCACGGTGTATGTTCCACCCCCAAATCCAGTTTCTAAAGTTGTGGCTACAG
ATGCTTATGTGACCCGGACGAACATCTTTTATCACGCTTCTTCTTCTCGACTCCTCGCCGTGGGACACCC
TTACTTTAGTATCAAGAGAGCAAACAAGACTGTGGTGCCCAAGGTCTCTGGTTACCAGTATAGAGTGTTC
AAGGTGGTGCTTCCTGACCCCAACAAGTTTGCCCTCCCTGATAGTAGTCTGTTCGATCCCACAACGCAAC
GACTGGTATGGGCGTGCACCGGTCTGGAAGTTGGTAGGGGGCAGCCACTCGGTGTAGGGGTTAGCGGCCA
CCCCTTTCTGAACAAGTATGATGACGTGGAAAACAGCGGAAGTGGAGGCAACCCTGGACAGGACAATCGG
GTAAACGTGGGCATGGACTATAAACAGACCCAGCTGTGCATGGTTGGGTGCGCCCCACCCCTGGGCGAAC
ACTGGGGTAAGGGCAAACAATGCACAAACACCCCTGTGCAGGCCGGCGACTGTCCACCTCTCGAACTTAT
CACCAGCGTCATTCAGGATGGCGATATGGTTGACACCGGTTTTGGTGCCATGAACTTCGCTGACCTCCAA
ACCAACAAATCTGACGTGCCAATAGATATCTGCGGCACAACCTGTAAGTATCCCGATTATCTGCAGATGG
CAGCCGATCCCTATGGTGATAGGCTGTTTTTTTTTCTGCGCAAAGAACAGATGTTTGCCCGGCATTTTTT
CAATAGAGCTGGCGAGGTGGGAGAACCAGTCCCAGACACACTGATCATAAAGGGCAGTGGAAATAGAACC
TCAGTTGGCTCTAGCATTTATGTGAATACCCCATCCGGATCACTGGTGTCTTCAGAGGCCCAGCTGTTCA
ACAAACCTTATTGGCTGCAGAAAGCACAGGGCCACAACAATGGCATATGCTGGGGCAACCAGCTGTTCGT
GACCGTTGTGGACACAACTCGCTCCACCAATATGACACTCTGCGCTAGCGTGACAACCAGCTCTACATAC
ACAAACAGCGACTACAAGGAATATATGCGGCACGTGGAAGAGTACGACCTTCAGTTCATATTCCAATTGT
GTAGTATTACCCTTTCTGCGGAGGTGATGGCTTACATCCATACAATGAACCCATCTGTACTCGAGGATTG
GAATTTCGGCCTGTCCCCACCACCAAACGGCACTCTGGAAGACACGTATCGATATGTCCAGAGTCAGGCC
ATCACTTGTCAAAAACCGACACCCGAGAAAGAGAAGCCCGATCCTTACAAGAACCTGTCTTTCTGGGAGG
TGAACCTGAAGGAGAAGTTCTCTAGTGAACTGGATCAGTACCCTCTGGGTAGAAAGTTCCTTTTGCAGTC
CGGATATAGGGGCAGGTCAAGTATCAGGACCGGAGTGAAAAGACCAGCAGTCAGTAAGGCATCAGCCGCA
CCTAAGCGGAAACGCGCCAAGACCAAACGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC102000 representing NP_040304
Red=Cloning sites Green=Tags

MWRPSDSTVYVPPPNPVSKVVATDAYVTRTNIFYHASSSRLLAVGHPYFSIKRANKTVVPKVSGYQYRVF
KVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHPFLNKYDDVENSGSGGNPGQDNR
VNVGMDYKQTQLCMVGCAPPLGEHWGKGKQCTNTPVQAGDCPPLELITSVIQDGDMVDTGFGAMNFADLQ
TNKSDVPIDICGTTCKYPDYLQMAADPYGDRLFFFLRKEQMFARHFFNRAGEVGEPVPDTLIIKGSGNRT
SVGSSIYVNTPSGSLVSSEAQLFNKPYWLQKAQGHNNGICWGNQLFVTVVDTTRSTNMTLCASVTTSSTY
TNSDYKEYMRHVEEYDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDTYRYVQSQA
ITCQKPTPEKEKPDPYKNLSFWEVNLKEKFSSELDQYPLGRKFLLQSGYRGRSSIRTGVKRPAVSKASAA
PKRKRAKTKR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001355
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001355.1, NP_040304
RefSeq ORF 1500 bp
Locus ID 1489370
MW 55.6 kDa
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"NC_001355" in other vectors (8)
SKU Description Size Price
VC101992 Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040296 10 ug
$289.00
VC101993 Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040297 10 ug
$289.00
VC101994 Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040298 10 ug
$664.00
VC101995 Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040299 10 ug
$503.00
VC101996 Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040300 10 ug
$165.00
VC101997 Myc-DDK-tagged ORF clone of viral ORF for E5a protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040301 10 ug
$165.00
VC101998 Myc-DDK-tagged ORF clone of viral ORF for E5b protein [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040302 10 ug
$165.00
VC101999 Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus type 6b], codon optimized for human cell expression, NP_040303 10 ug
$503.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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