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L1 (NC_001357) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC101810
Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040317
  $582.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC101810 represents NCBI reference of NP_040317 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTCTGTATACAAGAGTCTTGATCCTTCACTACCATCTCCTTCCTTTGTATGGGCCACTTTACCACC
CCAGGCCGCTGCCCCTTCACTCCATTTTGGTGTATATGGTCCATATAATAATCTGTGGGCATTATATTAT
ATTGTTTCTGCGAAATGTGAATGTTTTTCCTATCTTCCTCCAGATGGCACTGTGGAGGCCAAGTGATAAC
ACCGTTTACCTTCCACCCCCCTCCGTAGCACGAGTCGTCAATACAGACGATTATGTCACCCCCACCAGCA
TATTCTACCATGCCGGTTCTAGCAGACTGCTTACTGTCGGGAATCCGTACTTCCGCGTTCCAGCTGGGGG
TGGCAACAAACAGGACATTCCTAAGGTCTCAGCTTATCAATACCGGGTCTTTCGCGTTCAGTTGCCCGAT
CCAAATAAGTTCGGACTCCCCGATACCTCAATTTACAATCCGGAGACACAGAGATTGGTCTGGGCATGTG
CCGGCGTGGAAATTGGACGAGGCCAGCCATTGGGCGTGGGCCTGTCCGGCCACCCCTTTTATAATAAACT
GGACGACACAGAAAGTAGCCACGCTGCGACATCTAACGTTTCAGAGGATGTGCGCGATAACGTGAGTGTG
GACTACAAGCAGACTCAGTTGTGCATACTCGGATGTGCCCCCGCCATAGGTGAACATTGGGCCAAGGGGA
CTGCCTGTAAGTCAAGGCCCCTTTCACAGGGCGACTGTCCGCCTCTGGAGCTGAAGAACACTGTGCTCGA
GGACGGGGATATGGTCGACACCGGGTACGGAGCCATGGACTTTAGCACGCTGCAGGACACCAAATGTGAG
GTGCCTCTGGATATCTGCCAGTCTATTTGCAAATACCCAGATTACCTTCAGATGAGCGCAGACCCCTATG
GCGATAGCATGTTCTTCTGTCTGCGGAGGGAGCAGCTCTTTGCCCGGCATTTTTGGAACAGGGCCGGCAC
CATGGGAGATACAGTGCCCCAGAGTCTGTACATTAAAGGAACTGGTATGCCCGCATCTCCAGGGTCATGC
GTATATTCACCAAGCCCCTCTGGCAGCATCGTTACAAGCGACTCCCAGCTGTTCAATAAGCCCTACTGGC
TCCATAAGGCTCAGGGCCATAACAATGGAGTCTGCTGGCACAATCAGCTGTTCGTGACTGTCGTGGACAC
CACTCCTTCCACTAATCTGACAATATGCGCCTCAACCCAGAGCCCTGTCCCCGGGCAGTACGACGCCACA
AAATTCAAGCAATACTCAAGGCACGTCGAAGAGTACGACCTCCAGTTCATATTCCAGCTGTGTACAATAA
CCTTGACCGCCGATGTGATGTCCTATATACATTCTATGAACAGTTCTATCTTGGAGGACTGGAATTTCGG
GGTCCCCCCACCCCCTACAACCTCACTCGTTGATACGTACAGATTCGTGCAGTCAGTGGCCATTACCTGC
CAAAAGGACGCGGCACCAGCCGAAAATAAAGACCCCTATGATAAGCTCAAATTCTGGAACGTCGATCTCA
AGGAGAAATTCTCCCTTGACCTGGATCAGTATCCTCTTGGACGCAAATTCCTGGTGCAGGCCGGGTTGCG
ACGAAAGCCAACCATCGGCCCCAGAAAGCGCTCTGCCCCTTCTGCTACTACTAGCAGCAAGCCCGCCAAG
CGGGTGAGGGTGCGCGCCAGAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC101810 representing NP_040317
Red=Cloning sites Green=Tags

MCLYTRVLILHYHLLPLYGPLYHPRPLPLHSILVYMVHIIICGHYIILFLRNVNVFPIFLQMALWRPSDN
TVYLPPPSVARVVNTDDYVTPTSIFYHAGSSRLLTVGNPYFRVPAGGGNKQDIPKVSAYQYRVFRVQLPD
PNKFGLPDTSIYNPETQRLVWACAGVEIGRGQPLGVGLSGHPFYNKLDDTESSHAATSNVSEDVRDNVSV
DYKQTQLCILGCAPAIGEHWAKGTACKSRPLSQGDCPPLELKNTVLEDGDMVDTGYGAMDFSTLQDTKCE
VPLDICQSICKYPDYLQMSADPYGDSMFFCLRREQLFARHFWNRAGTMGDTVPQSLYIKGTGMPASPGSC
VYSPSPSGSIVTSDSQLFNKPYWLHKAQGHNNGVCWHNQLFVTVVDTTPSTNLTICASTQSPVPGQYDAT
KFKQYSRHVEEYDLQFIFQLCTITLTADVMSYIHSMNSSILEDWNFGVPPPPTTSLVDTYRFVQSVAITC
QKDAAPAENKDPYDKLKFWNVDLKEKFSLDLDQYPLGRKFLVQAGLRRKPTIGPRKRSAPSATTSSKPAK
RVRVRARK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001357
ORF Size 1704 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001357.1, NP_040317
RefSeq ORF 1704 bp
Locus ID 1489090
UniProt ID P06463
MW 63.6 kDa
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Documents
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"NC_001357" in other vectors (7)
SKU Description Size Price
VC101803 Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040310 10 ug
$289.00
VC101804 Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040311 10 ug
$165.00
VC101805 Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040312 10 ug
$673.00
VC101806 Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040313 10 ug
$503.00
VC101807 Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040314 10 ug
$165.00
VC101808 Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040315 10 ug
$165.00
VC101809 Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040316 10 ug
$503.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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