E7 (NC_001357) Virus Tagged ORF Clone

  • TrueORF®
SKU
VC101804
Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040311
  $165.00
3 Weeks*
Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol E7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC101804 represents NCBI reference of NP_040311 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATGGTCCAAAAGCTACCTTGCAGGACATTGTGTTGCATCTGGAACCGCAAAATGAGATCCCCGTCG
ACCTCCTGTGCCATGAACAACTCTCCGACAGCGAGGAGGAAAATGACGAGATTGATGGCGTTAACCACCA
GCATTTGCCAGCTAGGCGCGCTGAACCTCAACGCCACACAATGTTGTGTATGTGTTGCAAATGCGAAGCT
AGGATCGAGCTGGTCGTGGAATCTTCCGCCGATGACCTCCGCGCCTTCCAACAGCTGTTCCTGAACACAC
TCTCTTTCGTCTGCCCCTGGTGTGCGAGTCAGCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>VC101804 representing NP_040311
Red=Cloning sites Green=Tags

MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDGVNHQHLPARRAEPQRHTMLCMCCKCEA
RIELVVESSADDLRAFQQLFLNTLSFVCPWCASQQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001357
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001357.1, NP_040311
RefSeq ORF 315 bp
Locus ID 1489089
UniProt ID P06463
MW 12.0 kDa
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Resources
"NC_001357" in other vectors (7)
SKU Description Size Price
VC101803 Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040310 10 ug
$289.00
VC101805 Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040312 10 ug
$673.00
VC101806 Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040313 10 ug
$503.00
VC101807 Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040314 10 ug
$165.00
VC101808 Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040315 10 ug
$165.00
VC101809 Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040316 10 ug
$503.00
VC101810 Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040317 10 ug
$582.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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