HCoV-229E S Gene Tagged ORF Clone
- TrueORF®
SKU
VC100563
Myc-DDK-tagged ORF clone for surface glycoprotein [Human coronavirus 229E], codon optimized for human cell expression, NP_073551
$1,123.00
5 Weeks*
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
|---|---|
| Target Symbol | S Protein |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NC_002645 |
| ORF Size | 3519 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NC_002645.1, NP_073551 |
| RefSeq ORF | 3519 bp |
| Locus ID | 918758 |
| MW | 128.6 kDa |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NC_002645" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| VC100564 | Myc-DDK-tagged ORF clone for 4a protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073552 | 10 ug |
$165.00
|
|
| VC100565 | Myc-DDK-tagged ORF clone for 4b protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073553 | 10 ug |
$165.00
|
|
| VC100566 | Myc-DDK-tagged ORF clone for envelope protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073554 | 10 ug |
$165.00
|
|
| VC100567 | Myc-DDK-tagged ORF clone for membrane protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073555 | 10 ug |
$289.00
$300.00
|
|
| VC100568 | Myc-DDK-tagged ORF clone for nucleocapsid protein [Human coronavirus 229E], codon optimized for human cell expression, NP_073556 | 10 ug |
$686.00
|
|
Citations
LY6E Restricts Entry of Human Coronaviruses, Including Currently Pandemic SARS-CoV-2
Zhao, Zheng, Chen et al
J Virol (2020) 94 (18) DOI: 10.1128/JVI.00562-20
Zhao, Zheng, Chen et al
J Virol (2020) 94 (18) DOI: 10.1128/JVI.00562-20
AI Summary: In this study, OriGene products including the LY6E (RC208447) and GILT (RC217745) expression plasmids were used to investigate the roles of these proteins in restricting human coronavirus entry, demonstrating that overexpression of LY6E inhibits infection by multiple coronaviruses including SARS-CoV-2.
Proteolytic processing of Middle East respiratory syndrome coronavirus spikes expands virus tropism
Park, Li, Barlan et al
PNAS (2016) 113 (43), 12262-12267 DOI: 10.1073/pnas.1608147113
Park, Li, Barlan et al
PNAS (2016) 113 (43), 12262-12267 DOI: 10.1073/pnas.1608147113
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry-hDPP4-flag plasmid, which was used to express the human DPP4 receptor essential for analyzing MERS-CoV spike protein function.
Snippet: All constructs were confirmed by Sanger sequencing. Plasmids encoding C-terminal flagtagged hDPP4 (GenBank accession no. NM_001935, pCMV6- Entry-hDPP4-flag) were purchased from OriGene
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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