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Argonaute 2 (AGO2) (NM_001164623) Human Untagged Clone

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SKU
SC327227
AGO2 (untagged)-Human eukaryotic translation initiation factor 2C 2 (EIF2C2) transcript variant 2
  $832.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Argonaute 2
Synonyms CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327227 representing NM_001164623.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTACTCGGGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAAGGATATGCCTTC
AAGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAGAACAATCAAATTACAGGCCAATTTCTTCGAA
ATGGACATCCCCAAAATTGACATCTATCATTATGAATTGGATATCAAGCCAGAGAAGTGCCCGAGGAGA
GTTAACAGGGAAATCGTGGAACACATGGTCCAGCACTTTAAAACACAGATCTTTGGGGATCGGAAGCCC
GTGTTTGACGGCAGGAAGAATCTATACACAGCCATGCCCCTTCCGATTGGGAGGGACAAGGTGGAGCTG
GAGGTCACGCTGCCAGGAGAAGGCAAGGATCGCATCTTCAAGGTGTCCATCAAGTGGGTGTCCTGCGTG
AGCTTGCAGGCGTTACACGATGCACTTTCAGGGCGGCTGCCCAGCGTCCCTTTTGAGACGATCCAGGCC
CTGGACGTGGTCATGAGGCACTTGCCATCCATGAGGTACACCCCCGTGGGCCGCTCCTTCTTCACCGCG
TCCGAAGGCTGCTCTAACCCTCTTGGCGGGGGCCGAGAAGTGTGGTTTGGCTTCCATCAGTCCGTCCGG
CCTTCTCTCTGGAAAATGATGCTGAATATTGATGTGTCAGCAACAGCGTTTTACAAGGCACAGCCAGTA
ATCGAGTTTGTTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCC
CAAAGGGTAAAGTTTACCAAAGAAATTAAAGGTCTAAAGGTGGAGATAACGCACTGTGGGCAGATGAAG
AGGAAGTACCGCGTCTGCAATGTGACCCGGCGGCCCGCCAGTCACCAAACATTCCCGCTGCAGCAGGAG
AGCGGGCAGACGGTGGAGTGCACGGTGGCCCAGTATTTCAAGGACAGGCACAAGTTGGTTCTGCGCTAC
CCCCACCTCCCATGTTTACAAGTCGGACAGGAGCAGAAACACACCTACCTTCCCCTGGAGGTCTGTAAC
ATTGTGGCAGGACAAAGATGTATTAAAAAATTAACGGACAATCAGACCTCAACCATGATCAGAGCGACT
GCTAGGTCGGCGCCCGATCGGCAAGAAGAGATTAGCAAATTGATGCGAAGTGCAAGTTTCAACACAGAT
CCATACGTCCGTGAATTTGGAATCATGGTCAAAGATGAGATGACAGACGTGACTGGGCGGGTGCTGCAG
CCGCCCTCCATCCTCTACGGGGGCAGGAATAAAGCTATTGCGACCCCTGTCCAGGGCGTCTGGGACATG
CGGAACAAGCAGTTCCACACGGGCATCGAGATCAAGGTGTGGGCCATTGCGTGCTTCGCCCCCCAGCGC
CAGTGCACGGAAGTCCATCTGAAGTCCTTCACAGAGCAGCTCAGAAAGATCTCGAGAGACGCCGGCATG
CCCATCCAGGGCCAGCCGTGCTTCTGCAAATACGCGCAGGGGGCGGACAGCGTGGAGCCCATGTTCCGG
CACCTGAAGAACACGTATGCGGGCCTGCAGCTGGTGGTGGTCATCCTGCCCGGCAAGACGCCCGTGTAC
GCCGAGGTCAAGCGCGTGGGAGACACGGTGCTGGGGATGGCCACGCAGTGCGTGCAGATGAAGAACGTG
CAGAGGACCACGCCACAGACCCTGTCCAACCTCTGCCTGAAGATCAACGTCAAGCTGGGAGGCGTGAAC
AACATCCTGCTGCCCCAGGGCAGGCCGCCGGTGTTCCAGCAGCCCGTCATCTTTCTGGGAGCAGACGTC
ACTCACCCCCCCGCCGGGGATGGGAAGAAGCCCTCCATTGCCGCCGTGGTGGGCAGCATGGACGCCCAC
CCCAATCGCTACTGCGCCACCGTGCGCGTGCAGCAGCACCGGCAGGAGATCATACAAGACCTGGCCGCC
ATGGTCCGCGAGCTCCTCATCCAGTTCTACAAGTCCACGCGCTTCAAGCCCACCCGCATCATCTTCTAC
CGCGACGGTGTCTCTGAAGGCCAGTTCCAGCAGGTTCTCCACCACGAGTTGCTGGCCATCCGTGAGGCC
TGTATCAAGCTAGAAAAAGACTACCAGCCCGGGATCACCTTCATCGTGGTGCAGAAGAGGCACCACACC
CGGCTCTTCTGCACTGACAAGAACGAGCGGGGGACAAGCAGGCCTTCGCACTATCACGTCCTCTGGGAC
GACAATCGTTTCTCCTCTGATGAGCTGCAGATCCTAACCTACCAGCTGTGTCACACCTACGTGCGCTGC
ACACGCTCCGTGTCCATCCCAGCGCCAGCATACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCAC
CTGGTGGATAAGGAACATGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGGGCGAGACCAC
CAAGCACTGGCCAAGGCGGTCCAGGTTCACCAAGACACTCTGCGCACCATGTACTTTGCTTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001164623
Insert Size 2478 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164623.1
RefSeq Size 3418 bp
RefSeq ORF 2478 bp
Locus ID 27161
UniProt ID Q9UKV8
Cytogenetics 8q24.3
Protein Families Druggable Genome
MW 93.6 kDa
Summary This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001164623" in other vectors (6)
SKU Description Size Price
RC228592 AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2 10 ug
$755.00
RC228592L1 Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,055.00
RC228592L2 Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged 10 ug
$1,055.00
RC228592L3 Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,055.00
RC228592L4 Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged 10 ug
$1,055.00
RG228592 AGO2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2 10 ug
$955.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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