Argonaute 2 (AGO2) (NM_001164623) Human Tagged ORF Clone
SKU
RC228592
AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2
$755.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Argonaute 2 |
| Synonyms | CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001164623 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001164623.3 |
| RefSeq ORF | 2478 bp |
| Locus ID | 27161 |
| UniProt ID | Q9UKV8 |
| Cytogenetics | 8q24.3 |
| Protein Families | Druggable Genome |
| MW | 93.4 kDa |
| Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001164623" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC228592L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,055.00
|
|
| RC228592L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged | 10 ug |
$1,055.00
|
|
| RC228592L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,055.00
|
|
| RC228592L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged | 10 ug |
$1,055.00
|
|
| RG228592 | AGO2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2 | 10 ug |
$955.00
|
|
| SC327227 | AGO2 (untagged)-Human eukaryotic translation initiation factor 2C 2 (EIF2C2) transcript variant 2 | 10 ug |
$832.00
|
|
Citations
circEFR3A promotes breast cancer progression by sponging miR-590-3p to upregulate androgen receptor expression
Mi, Wang, Song et al
Oncol Lett (2025) 30 (3), 446 DOI: 10.3892/ol.2025.15192
Mi, Wang, Song et al
Oncol Lett (2025) 30 (3), 446 DOI: 10.3892/ol.2025.15192
Cited by 1
AI Summary: OriGene’s Myc-tagged AGO2 vector (cat. no. RC228592) was used in RNA immunoprecipitation assays to investigate the interaction between circEFR3A, miR-590-3p, and androgen receptor expression in breast cancer cells.
Snippet: vity was standardized by comparison with Renilla luciferase activity. RNA immunoprecipitation (RIP) assay MDA-MB-231 cells were co-transfected with a Myc-tagged AGO2 vector (1 µg;cat. no. RC228592;OriGene Technologies, Inc.) and miR-590-3p mimics (50 nM) using Lipofectamine 3000 transfection reagent according to the manufacturer's protocol. Cells were maintained at 37°C in a 5% CO2 incubator d
Showing 1-1 of 1 papers.
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