Argonaute 2 (AGO2) (NM_001164623) Human Tagged ORF Clone
- TrueORF®
SKU
RG228592
AGO2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2
$955.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Argonaute 2 |
| Synonyms | CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001164623 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001164623.3 |
| RefSeq Size | 3418 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 27161 |
| UniProt ID | Q9UKV8 |
| Cytogenetics | 8q24.3 |
| Protein Families | Druggable Genome |
| Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001164623" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC228592 | AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2 | 10 ug |
$755.00
|
|
| RC228592L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,055.00
|
|
| RC228592L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged | 10 ug |
$1,055.00
|
|
| RC228592L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,055.00
|
|
| RC228592L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 2, mGFP tagged | 10 ug |
$1,055.00
|
|
| SC327227 | AGO2 (untagged)-Human eukaryotic translation initiation factor 2C 2 (EIF2C2) transcript variant 2 | 10 ug |
$832.00
|
|
Citations
The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
Kratassiouk, Pritchard, Cuvellier et al
Cell Cycle (2016) 15 (5), 667-77 DOI: 10.1080/15384101.2016.1147631
Kratassiouk, Pritchard, Cuvellier et al
Cell Cycle (2016) 15 (5), 667-77 DOI: 10.1080/15384101.2016.1147631
Cited by 7
miR-20a represses endothelial cell migration by targeting MKK3 and inhibiting p38 MAP kinase activation in response to VEGF
Pin, Houle, Guillonneau et al
Angiogenesis (2012) 15 (4), 593-608 DOI: 10.1007/s10456-012-9283-z
Pin, Houle, Guillonneau et al
Angiogenesis (2012) 15 (4), 593-608 DOI: 10.1007/s10456-012-9283-z
Cited by 57
AI Summary: OriGene contributed to this study by providing the Human Ago2 expressing plasmid, which was used to investigate the role of Argonaute 2 in miRNA-mediated regulation of VEGF-induced endothelial cell migration.
Snippet: DiGeorge syndrome critical region gene 8 protein) and the RNase III enzyme Drosha, that form the microprocessor complex, process long primary miRNAs (pri-miRNAs) into short hairpins called ... Human Ago2 expressing plasmid was obtained from OriGene (Rockville, MD, USA
Showing 1-2 of 2 papers.
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