KLF1 (NM_006563) Human Untagged Clone

SKU
SC323869
KLF1 (untagged)-Human Kruppel-like factor 1 (erythroid) (KLF1)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KLF1
Synonyms EKLF; EKLF/KLF1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_006563.2 CTTGAGGCCCAGGGTGGGCACCAGCCAGCCATGGCCACAGCCGAGACCGCCTTGCCCTCC
ATCAGCACACTGACCGCCCTGGGCCCCTTCCCGGACACACAGGATGACTTCCTCAAGTGG
TGGCGCTCCGAAGAGGCGCAGGACATGGGCCCGGGTCCTCCTGACCCCACGGAGCCGCCC
CTCCACGTGAAGTCTGAGGACCAGCCCGGGGAGGAAGAGGACGATGAGAGGGGCGCGGAC
GCCACCTGGGACCTGGATCTCCTCCTCACCAACTTCTCGGGCCCGGAGCCCGGTGGCGCG
CCCCAGACCTGCGCTCTGGCGCCCAGCGAGGCCCCCGGGGCGCAATATCCGCCGCCGCCC
GAGACTCTGGGCGCATATGCTGGCGGCCCGGGGCTGGTGGCTGGGCTTTTGGGTTCGGAG
GATCACTCGGGTTGGGTGCGCCCTGCCCTGCGAGCCCGGGCTCCCGACGCCTTCGTGGGC
CCAGCCCTGGCTCCAGCCCCGGCCCCCGAGCCCAAGGCGCTGGCGCTGCAACCGGTGTAC
CCGGGGCCCGGCGCCGGCTCCTCGGGTGGCTACTTCCCGCGGACCGGGCTTTCAGTGCCT
GCGGCGTCGGGCGCCCCCTACGGGCTACTGTCCGGGTACCCCGCGATGTACCCGGCGCCT
CAGTACCAAGGGCACTTCCAGCTCTTCCGCGGGCTCCAGGGACCCGCGCCCGGTCCCGCC
ACGTCCCCCTCCTTCCTGAGTTGTTTGGGACCCGGGACGGTGGGCACTGGACTCGGGGGG
ACTGCAGAGGATCCAGGTGTGATAGCCGAGACCGCGCCATCCAAGCGAGGCCGACGTTCG
TGGGCGCGCAAGAGGCAGGCAGCGCACACGTGCGCGCACCCGGGTTGCGGCAAGAGCTAC
ACCAAGAGCTCCCACCTGAAGGCGCATCTGCGCACGCACACAGGGGAGAAGCCATACGCC
TGCACGTGGGAAGGCTGCGGCTGGAGATTCGCGCGCTCGGACGAGCTGACCCGCCACTAC
CGGAAACACACGGGGCAGCGCCCCTTCCGCTGCCAGCTCTGCCCACGTGCTTTTTCGCGC
TCTGACCACCTGGCCTTGCACATGAAGCGCCACCTTTGAGCCCTGCCCTGGCACTTGGAC
TCTCCTAGTGACTGGGGATGGGACAAGAAGCCTGTTTGGTGGTCTCTTCACACGGACGCG
CGTGACACAATGCTGGGTGGTTTTCCCACGAATGGACCCTCTCCTGGACTCGCGTTCCCA
AAGATCCACCCAAATATCAAACACGGACCCATAGACAGCCCTGGGGGAGCCTCTTACGGA
AAATCCGACAAGCCTTCAGCCACAGGGAGCCACACAGAGATGTCCAAACTGTCGTGCAAA
CCCAGTGAGACAGACCGCCAAATAAACGGACTCAGTGGACACTCAGACCAGCTCCCAGAT
GGCCCTGGACAGCAGGAGAGGGTGTGGGATGAGGCTTCCCAGAGACCCTGGGTCTAGAAA
GCGGCTCCTGAAGGTCCCTTATTGTGGCTGATATTAACTGTCAATGGTTATGGGTCCTAT
AAAAATGCCCCTCCCAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_006563
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006563.2, NP_006554.1
RefSeq Size 1618 bp
RefSeq ORF 1089 bp
Locus ID 10661
UniProt ID Q13351
Cytogenetics 19p13.13
Protein Families Transcription Factors
Summary This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_006563" in other vectors (6)
SKU Description Size Price
RC206452 KLF1 (Myc-DDK-tagged)-Human Kruppel-like factor 1 (erythroid) (KLF1) 10 ug
$289.00 $457.00
RC206452L1 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged 10 ug
$757.00
RC206452L2 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged 10 ug
$757.00
RC206452L3 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged 10 ug
$757.00
RC206452L4 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged 10 ug
$757.00
RG206452 KLF1 (tGFP-tagged) - Human Kruppel-like factor 1 (erythroid) (KLF1) 10 ug
$489.00 $657.00

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