KLF1 Goat Polyclonal Antibody

Snippet: antibody-bead complexes were incubated with cell lysates at 4 C overnight with rotation. The antibodies used were mouse anti-RNA Pol II (Diagenode C15100055-100 RRID: AB_2750842) and goat anti-KLF1 (Origene TA305808). The beads were retrieved using a magnetic stand and rinsed with RIPA buffer. Elution buffer containing 50 mM Tris–HCl, pH 8;10 mM EDTA;1% SDS was added to the beads for reverse

Snippet: … Immunoprecipitations were performed using 100 µl of Dynabeads Protein G (ThermoFisher Scientific) complexed to 15 µg of KLF1 antibody (OriGene, #TA305808) or normal rabbit IgG (Cell Signaling Technology #2729S) at 4°C overnight. Magnetic beads were separated and washed thoroughly before elution and cross-linking was reversed by incu;Immunoprecipitations were performed using 100 µl of Dynabeads Protein G (ThermoFisher Scientific) complexed to 15 µg of KLF1 antibody (OriGene, #TA305808)