OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

KLF1 (NM_006563) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RC206452
KLF1 (Myc-DDK-tagged)-Human Kruppel-like factor 1 (erythroid) (KLF1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA368227 KLF1 rabbit polyclonal antibody
100 ul
$310.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "KLF1 or NM_006563"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLF1
Synonyms EKLF; EKLF/KLF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206452 representing NM_006563
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACAGCCGAGACCGCCTTGCCCTCCATCAGCACACTGACCGCCCTGGGCCCCTTCCCGGACACAC
AGGATGACTTCCTCAAGTGGTGGCGCTCCGAAGAGGCGCAGGACATGGGCCCGGGTCCTCCTGACCCCAC
GGAGCCGCCCCTCCACGTGAAGTCTGAGGACCAGCCCGGGGAGGAAGAGGACGATGAGAGGGGCGCGGAC
GCCACCTGGGACCTGGATCTCCTCCTCACCAACTTCTCGGGCCCGGAGCCCGGTGGCGCGCCCCAGACCT
GCGCTCTGGCGCCCAGCGAGGCCTCCGGGGCGCAATATCCGCCGCCGCCCGAGACTCTGGGCGCATATGC
TGGCGGCCCGGGGCTGGTGGCTGGGCTTTTGGGTTCGGAGGATCACTCGGGTTGGGTGCGCCCTGCCCTG
CGAGCCCGGGCTCCCGACGCCTTCGTGGGCCCAGCCCTGGCTCCAGCCCCGGCCCCCGAGCCCAAGGCGC
TGGCGCTGCAACCGGTGTACCCGGGGCCCGGCGCCGGCTCCTCGGGTGGCTACTTCCCGCGGACCGGGCT
TTCAGTGCCTGCGGCGTCGGGCGCCCCCTACGGGCTACTGTCCGGGTACCCCGCGATGTACCCGGCGCCT
CAGTACCAAGGGCACTTCCAGCTCTTCCGCGGGCTCCAGGGACCCGCGCCCGGTCCCGCCACGTCCCCCT
CCTTCCTGAGTTGTTTGGGACCCGGGACGGTGGGCACTGGACTCGGGGGGACTGCAGAGGATCCAGGTGT
GATAGCCGAGACCGCGCCATCCAAGCGAGGCCGACGTTCGTGGGCGCGCAAGAGGCAGGCAGCGCACACG
TGCGCGCACCCGGGTTGCGGCAAGAGCTACACCAAGAGCTCCCACCTGAAGGCGCATCTGCGCACGCACA
CAGGGGAGAAGCCATACGCCTGCACGTGGGAAGGCTGCGGCTGGAGATTCGCGCGCTCGGACGAGCTGAC
CCGCCACTACCGGAAACACACGGGGCAGCGCCCCTTCCGCTGCCAGCTCTGCCCACGTGCTTTTTCGCGC
TCTGACCACCTGGCCTTGCACATGAAGCGCCACCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206452 representing NM_006563
Red=Cloning site Green=Tags(s)

MATAETALPSISTLTALGPFPDTQDDFLKWWRSEEAQDMGPGPPDPTEPPLHVKSEDQPGEEEDDERGAD
ATWDLDLLLTNFSGPEPGGAPQTCALAPSEASGAQYPPPPETLGAYAGGPGLVAGLLGSEDHSGWVRPAL
RARAPDAFVGPALAPAPAPEPKALALQPVYPGPGAGSSGGYFPRTGLSVPAASGAPYGLLSGYPAMYPAP
QYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGGTAEDPGVIAETAPSKRGRRSWARKRQAAHT
CAHPGCGKSYTKSSHLKAHLRTHTGEKPYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSR
SDHLALHMKRHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006563
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006563.5
RefSeq Size 1618 bp
RefSeq ORF 1089 bp
Locus ID 10661
UniProt ID Q13351
Cytogenetics 19p13.13
Protein Families Transcription Factors
MW 38 kDa
Summary This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. provided by RefSeq, Oct 2009
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_006563" in other vectors (6)
SKU Description Size Price
RC206452L1 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged 10 ug
$757.00
RC206452L2 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged 10 ug
$757.00
RC206452L3 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), Myc-DDK-tagged 10 ug
$757.00
RC206452L4 Lenti ORF clone of Human Kruppel-like factor 1 (erythroid) (KLF1), mGFP tagged 10 ug
$757.00
RG206452 KLF1 (tGFP-tagged) - Human Kruppel-like factor 1 (erythroid) (KLF1) 10 ug
$489.00 $657.00
SC323869 KLF1 (untagged)-Human Kruppel-like factor 1 (erythroid) (KLF1) 10 ug
$686.00

Plasmid Map for RC206452

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206452
Circular map for RC206452

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products