COX2 (PTGS2) (NM_000963) Human Untagged Clone
SKU
SC323764
PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2)
$844.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | COX2 |
| Synonyms | COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | ECoRI-NOT | |
| ACCN | NM_000963 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000963.1, NP_000954.1 |
| RefSeq Size | 4465 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 5743 |
| UniProt ID | P35354 |
| Cytogenetics | 1q31.1 |
| Domains | An_peroxidase, EGF |
| Protein Families | Druggable Genome |
| Protein Pathways | Arachidonic acid metabolism, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
| Summary | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. provided by RefSeq, Feb 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000963" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202245 | PTGS2 (Myc-DDK-tagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) | 10 ug |
$289.00
$843.00
|
|
| RC202245L1 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged | 10 ug |
$1,143.00
|
|
| RC202245L2 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged | 10 ug |
$1,143.00
|
|
| RC202245L3 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged | 10 ug |
$1,143.00
|
|
| RC202245L4 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged | 10 ug |
$1,143.00
|
|
| RG202245 | PTGS2 (tGFP-tagged) - Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) | 10 ug |
$489.00
$1,043.00
|
|
| SC128243 | PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) | 10 ug |
$844.00
|
|
Citations
Cyclosporine A binding to COX-2 reveals a novel signaling pathway that activates the IRE1α unfolded protein response sensor
Groenendyk, Paskevicius, Urra et al
Sci Rep (2018) 8 (1), 16678 DOI: 10.1038/s41598-018-34891-w
Groenendyk, Paskevicius, Urra et al
Sci Rep (2018) 8 (1), 16678 DOI: 10.1038/s41598-018-34891-w
AI Summary: In this study, OriGene's Cyclophilin A and carbonic anhydrase proteins were used to investigate protein interactions relevant to the newly discovered cyclosporine-COX-2-IRE1α signaling pathway.
Snippet: mbion and Dharmafect Duo was from GElifesciences. Cyclosporine, thapsigargin, tunicamycin, DTT, celecoxib and other chemicals were from Sigma. Cyclophilin A and carbonic anhydrase were purchased from OriGene. siRNA library screen A genome wide druggable library screen (400 genes) was carried out in NIH-3T3 mouse fibroblast cells as described previously31. Briefly, NIH-3T3 cells were transfected w...
MicroRNA-143-3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase-2 and inversely correlates with malignant melanoma progression
Panza, Ercolano, De Cicco et al
Biochem. Pharmacol. (2018) 156, 52-59 DOI: 10.1016/j.bcp.2018.08.008
Panza, Ercolano, De Cicco et al
Biochem. Pharmacol. (2018) 156, 52-59 DOI: 10.1016/j.bcp.2018.08.008
Molecular pathways reflecting poor intrauterine growth are found in Wharton's jelly-derived mesenchymal stem cells
Sukarieh, Joseph, Leow et al
Hum. Reprod. (2014)
Sukarieh, Joseph, Leow et al
Hum. Reprod. (2014)
Cited by 24
COX-2 and fatty acid amide hydrolase can regulate the time course of depolarization-induced suppression of excitation
Straiker, Wager-Miller, Hu et al
Br. J. Pharmacol. (2011) 164 (6), 1672-83 DOI: 10.1111/j.1476-5381.2011.01486.x
Straiker, Wager-Miller, Hu et al
Br. J. Pharmacol. (2011) 164 (6), 1672-83 DOI: 10.1111/j.1476-5381.2011.01486.x
Cited by 59
AI Summary: OriGene Technologies contributed the Human Cox2pCMV6-AC plasmid (Cat no. [not specified]) to this study, enabling the authors to overexpress COX-2 in neurons and investigate its role in regulating the duration of depolarization-induced suppression of excitation (DSE).
Snippet: Neurons were obtained from animals (age postnatal day 0–2) and plated onto a feeder layer of hippocampal astrocytes that had been laid down previously (Levison and McCarthy, 1991). ... Human Cox2pCMV6-AC plasmid was obtained from Origene Technologies (Cat no.
Showing 1-4 of 4 papers.
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