COX2 (PTGS2) (NM_000963) Human Tagged Lenti ORF Clone

SKU
RC202245L4
Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,143.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COX2
Synonyms COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC202245).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_000963
ORF Size 1812 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000963.1
RefSeq Size 4465 bp
RefSeq ORF 1815 bp
Locus ID 5743
UniProt ID P35354
Cytogenetics 1q31.1
Domains An_peroxidase, EGF
Protein Families Druggable Genome
Protein Pathways Arachidonic acid metabolism, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway
MW 69 kDa
Summary Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
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SKU Description Size Price
RC202245 PTGS2 (Myc-DDK-tagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) 10 ug
$289.00 MSRP $843.00 MSRP $843.00
RC202245L1 Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged 10 ug
$1,143.00
RC202245L2 Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged 10 ug
$1,143.00
RC202245L3 Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged 10 ug
$1,143.00
RG202245 PTGS2 (tGFP-tagged) - Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) 10 ug
$489.00 MSRP $1,043.00 MSRP $1,043.00
SC128243 PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) 10 ug
$844.00
SC323764 PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) 10 ug
$844.00

Citations

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