ABCG2 (NM_004827) Human Untagged Clone
$916.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | ABCG2 |
| Synonyms | ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_004827 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004827.2, NP_004818.2 |
| RefSeq Size | 4445 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 9429 |
| UniProt ID | Q9UNQ0 |
| Cytogenetics | 4q22.1 |
| Domains | AAA, ABC_tran |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | ABC transporters |
| Summary | The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2012 Transcript Variant: This variant (1) encodes the longest isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004827" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205640 | ABCG2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$289.00
$914.00
|
|
| RC205640L1 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged | 10 ug |
$1,214.00
|
|
| RC205640L2 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged | 10 ug |
$1,214.00
|
|
| RC205640L3 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged | 10 ug |
$1,214.00
|
|
| RC205640L4 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged | 10 ug |
$1,214.00
|
|
| RG205640 | ABCG2 (tGFP-tagged) - Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$489.00
$1,114.00
|
|
| SC117127 | ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$916.00
|
|
Citations
Contrasting roles of the ABCG2 Q141K variant in prostate cancer
Sobek, Cummings, Bacich et al
Exp. Cell Res. (2017)
Sobek, Cummings, Bacich et al
Exp. Cell Res. (2017)
AI Summary: OriGene’s pCMV6-AC plasmid containing ABCG2 cDNA was used as the source for generating wild-type and variant ABCG2 constructs, enabling functional studies of the Q141K variant’s impact on prostate cancer progression and drug response.
Snippet: 2.3. Lentiviral transduction and site-directed mutagenesis. ABCG2 cDNA was excised with XhoI and EcoRI, from the pCMV6-AC plasmid (Origene, Rockville, MD) and sub-cloned with BamHI and EcoRI into the lentiviral vector, pLVX-puro (Clontech, Mountain View, ... All 2 versions Cite SaveSaving
A role for ABCG2 beyond drug transport: regulation of autophagy
Ding, Jin, Pabon et al
Autophagy (2016)
Ding, Jin, Pabon et al
Autophagy (2016)
Cited by 58
AI Summary: OriGene’s pCMV-ABCG2 plasmid (SC320948) was used to overexpress ABCG2 in MCF-7 cells, enabling the study of ABCG2’s role in regulating autophagy and stress resistance in this research.
Snippet: MCF-7 cells were seeded at 2.5×105 per well in 6-well plates and cultured overnight before transfection. 2 µg of plasmid pCMV-V6 (empty vector control) or pCMV-ABCG2 (Origene, SC320948) were mixed with Lipofectamine 2000 (Life Technologies, 11668027) at a 1:3 ratio according to the manufacturer's instructions and added to the culture media. …
Glutathione transport is a unique function of the ATP-binding cassette protein ABCG2
Brechbuhl, Gould, Kachadourian et al
J Biol Chem (2010) 285 (22), 16582-7 DOI: 10.1074/jbc.M109.090506
Brechbuhl, Gould, Kachadourian et al
J Biol Chem (2010) 285 (22), 16582-7 DOI: 10.1074/jbc.M109.090506
Vliv alelických variant transportéru ABCG2 na transport kyseliny močové
Vavra
Thesis (2019)
Vavra
Thesis (2019)
Snippet: ABCG2 - plazmid pCMV6-AC ABCG2, Origene SC320948 URAT1 - plazmid pcDNA3 CMV SLC22A12, Invitrogen. Primery - IDT Integrated DNA technologies, Leuven, Belgie. Restrikční endonukleázy EcoR1 a Xho1 - Fast digest, Theromfisher. DNA marker Gene Ruler 1 kb DNA ladder - Thermo Scientific. Ampicilin - zásobní vodný roztok o koncentraci 100 mg/ml
Showing 1-4 of 4 papers.
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