ABCG2 (NM_004827) Human Tagged ORF Clone
- TrueORF®
SKU
RG205640
ABCG2 (tGFP-tagged) - Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)
$489.00
$1,114.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ABCG2 |
| Synonyms | ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004827 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004827.3 |
| RefSeq Size | 4445 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 9429 |
| UniProt ID | Q9UNQ0, A1LUE4 |
| Cytogenetics | 4q22.1 |
| Domains | AAA, ABC_tran |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | ABC transporters |
| Summary | The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004827" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205640 | ABCG2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$289.00
$914.00
|
|
| RC205640L1 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged | 10 ug |
$1,214.00
|
|
| RC205640L2 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged | 10 ug |
$1,214.00
|
|
| RC205640L3 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged | 10 ug |
$1,214.00
|
|
| RC205640L4 | Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged | 10 ug |
$1,214.00
|
|
| SC117127 | ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$916.00
|
|
| SC320948 | ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) | 10 ug |
$916.00
|
|
Citations
Correlation between Membrane Protein Expression Levels and Transcellular Transport Activity for Breast Cancer Resistance Protein
Liu, Huang, Li et al
Drug Metab. Dispos. (2017) 45 (5), 449-456 DOI: 10.1124/dmd.116.074245
Liu, Huang, Li et al
Drug Metab. Dispos. (2017) 45 (5), 449-456 DOI: 10.1124/dmd.116.074245
AI Summary: OriGene contributed to this study by providing the Abcg2 murine cDNA clone, which was used to express murine BCRP1 in MDCKII cells for investigating the correlation between BCRP expression levels and transcellular transport activity.
Snippet: The BCRP sequence was verified in house. Abcg2 murine cDNA clone was purchased from Origene (Rockville, MD). PCR cloning of human BCRP or murine Bcrp1 cDNA into pHTBV1mcs3 vector between BamHI and XhoI sites was verified View as HTML
Showing 1-1 of 1 papers.
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