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ABCG2 (NM_004827) Human Untagged Clone

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SKU
SC117127
ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2)
  $916.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ABCG2
Synonyms ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_004827 edited
TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGG
ACCGAGCTCTATTAAGCTGAAAAGATAAAAACTCTCCAGATGTCTTCCAGTAATGTCGAA
GTTTTTATCCCAGTGTCACAAGGAAACACCAATGGCTTCCCCGCGACAGCTTCCAATGAC
CTGAAGGCATTTACTGAAGGAGCTGTGTTAAGTTTTCATAACATCTGCTATCGAGTAAAA
CTGAAGAGTGGCTTTCTACCTTGTCGAAAACCAGTTGAGAAAGAAATATTATCGAATATC
AATGGGATCATGAAACCTGGTCTCAACGCCATCCTGGGACCCACAGGTGGAGGCAAATCT
TCGTTATTAGATGTCTTAGCTGCAAGGAAAGATCCAAGTGGATTATCTGGAGATGTTCTG
ATAAATGGAGCACCGCGACCTGCCAATTTCAAATGTAATTCAGGTTACGTGGTACAAGAT
GATGTTGTGATGGGCACTCTGACGGTGAGAGAAAACTTACAGTTCTCAGCAGCTCTTCGG
CTTGCAACAACTATGACGAATCATGAAAAAAACGAACGGATTAACAGGGTCATTCAAGAG
TTAGGTCTGGATAAAGTGGCAGACTCCAAGGTTGGAACTCAGTTTATCCGTGGTGTGTCT
GGAGGAGAAAGAAAAAGGACTAGTATAGGAATGGAGCTTATCACTGATCCTTCCATCTTG
TTCTTGGATGAGCCTACAACTGGCTTAGACTCAAGCACAGCAAATGCTGTCCTTTTGCTC
CTGAAAAGGATGTCTAAGCAGGGACGAACAATCATCTTCTCCATTCATCAGCCTCGATAT
TCCATCTTCAAGTTGTTTGATAGCCTCACCTTATTGGCCTCAGGAAGACTTATGTTCCAC
GGGCCTGCTCAGGAGGCCTTGGGATACTTTGAATCAGCTGGTTATCACTGTGAGGCCTAT
AATAACCCTGCAGACTTCTTCTTGGACATCATTAATGGAGATTCCACTGCTGTGGCATTA
AACAGAGAAGAAGACTTTAAAGCCACAGAGATCATAGAGCCTTCCAAGCAGGATAAGCCA
CTCATAGAAAAATTAGCGGAGATTTATGTCAACTCCTCCTTCTACAAAGAGACAAAAGCT
GAATTACATCAACTTTCCGGGGGTGAGAAGAAGAAGAAGATCACAGTCTTCAAGGAGATC
AGCTACACCACCTCCTTCTGTCATCAACTCAGATGGGTTTCCAAGCGTTCATTCAAAAAC
TTGCTGGGTAATCCCCAGGCCTCTATAGCTCAGATCATTGTCACAGTCGTACTGGGACTG
GTTATAGGTGCCATTTACTTTGGGCTAAAAAATGATTCTACTGGAATCCAGAACAGAGCT
GGGGTTCTCTTCTTCCTGACGACCAACCAGTGTTTCAGCAGTGTTTCAGCCGTGGAACTC
TTTGTGGTAGAGAAGAAGCTCTTCATACATGAATACATCAGCGGATACTACAGAGTGTCA
TCTTATTTCCTTGGAAAACTGTTATCTGATTTATTACCCATGAGGATGTTACCAAGTATT
ATATTTACCTGTATAGTGTACTTCATGTTAGGATTGAAGCCAAAGGCAGATGCCTTCTTC
GTTATGATGTTTACCCTTATGATGGTGGCTTATTCAGCCAGTTCCATGGCACTGGCCATA
GCAGCAGGTCAGAGTGTGGTTTCTGTAGCAACACTTCTCATGACCATCTGTTTTGTGTTT
ATGATGATTTTTTCAGGTCTGTTGGTCAATCTCACAACCATTGCATCTTGGCTGTCATGG
CTTCAGTACTTCAGCATTCCACGATATGGATTTACGGCTTTGCAGCATAATGAATTTTTG
GGACAAAACTTCTGCCCAGGACTCAATGCAACAGGAAACAATCCTTGTAACTATGCAACA
TGTACTGGCGAAGAATATTTGGTAAAGCAGGGCATCGATCTCTCACCCTGGGGCTTGTGG
AAGAATCACGTGGCCTTGGCTTGTATGATTGTTATTTTCCTCACAATTGCCTACCTGAAA
TTGTTATTTCTTAAAAAATATTCTTAAATTTCCCCTTAATTCAGTATGATTTATCCTCAC
ATAAAAAAGAAGCACTTTGATTGAAGTATTCAATCAAGTTTTTTTGTTGTTTTCTGTTCC
CTTGCCA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004827 unedited
NGGCCGCTCAGGATTTTCGTAAACGACTTACTATAGGCGGCCGCGAATTCGCACGAGAAC
CGAGCTCTATTAAGCTGAAAAAATAAAAACTCTCCAGATGTCTTCCAGTAATGTCGAAGT
TTTTATCCCAGTGTCACAAGGAAACACCAATGGCTTCCCCGCGACAGCTTCCAATGACCT
GAAGGCATTTACTGAAGGAGCTGTGTTAAGTTTTCATAACATCTGCTATCGAGTAAAACT
GAAGAGTGGCTTTCTACCTTGTCGAAAACCAGTTGAGAAAGAAATATTATCGAATATCAA
TGGGATCATGAAACCTGGTCTCAACGCCATCCTGGGACCCACAGGTGGAGGCAAATCTTC
GTTATTAGATGTCTTAGCTGCAAGGAAAGATCCAAGTGGATTATCTGGAGATGTTCTGAT
AAATGGAGCACCGCGACCTGCCAATTTCAAATGTAATTCAGGTTACGTGGTACAAGATGA
TGTTGTGATGGGCACTCTGACGGTGAGAGAAAACTTACAGTTCTCAGCAGCTCTTCGGCT
TGCAACAACTATGACGAATCATGAAAAAAACGAACGGATTAACAGGGTCATTCAAGAGTT
AGGTCTGGATAAAGTGGCAGACTCCAAGGTTGGAACTCAGTTTATCCGTGGTGTGTCTGG
AGGAGAAAGAAAAAGGACTAGTATAGGAATGGAGCTTATCACTGATCCTTCCATCTTGTT
CTTGGATGAGCCTACAACTGGCTTAGACTCAAGCACAGCANATGCTGTCCTTTTGCTCCT
GAAAGGATGTCTAAGCAGGNACGAACAATCATCTTCTCCATTCATCAGCCTCGATATTCC
ATCTTCAAGTTGTTGATAGCCTCACCTTATTNGCCTCANGAAGACTTATGNTNCACGGNC
CCTGCTCAGNAGCCTTNGGGATCTTTGAAATCAGCTGTTATCACTGTGGAGGCCTATAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004827 unedited
ACTTGGACGCGGCCGCATCTATGATCAGTTTTTTTTTTTTTTTTTTTAAGTTCTATTAAC
TTTAATGTGCTTATCAAAAAGATTTTTTTCTATCACGGAGGCTTTTTTTACCCTATAAGA
CAGAATCTTTTTTCCAACATATTTTCAATTTTACATTTCCAGTATAGCATTAATACATTT
GTCATTTATTAAATAATGGCAAACCATAGCATCATAAAGTATTAAGACTGAAGGGCTACT
AACCTACCTATTCATTTTAAAGATGAGGAAACAAATGCCAGAGACAGTAATAACTTGTCA
GGAGTTTCCAGAATTCAATTCTCCTTTTTTAGATTAATAAATTAGACCAGATTTCTTCCC
CATGGTTACTGTCTGAGGAGATTAACTAATATGCGATACATGATGTCTTGGGTTCTTTCA
TGTTTAATTCAGTTTGTTGAGATTTCTAAAAATGTATCTCTTTAAAACAATTGCTGCTGT
GCAACAGTGTGATGGCAAGGGAACAGAAAACAACAAAAAAACTTGATTGAATACTTCAAT
CAAAGTGCTTCTTTTTTATGTGAGGATAAATCATACTGAATTAAGGGGAAATTTAAGAAT
ATTTTTTAAGAAATAACAATTTCAGGTAGGCAATTGTGAGGAAAATAACAATCATACAAG
CCAAGGCCACGTGATTCTTCCACAAGCCCCAGGGTGAGAGATCGATGCCCTGCTTTACCA
AATATTCTTCGCCAGTACATGTTGCATAGTTACAAAGATTGTTTCCTGTTGCATTGAGTC
CTGAGCAGAAGTTTTGTCCCAAAATCCATTATGCTGCAAAGCCGTAAATCCATATCGNGG
AATGCTCGAGTACTGGAGCCATGACAGNCCAGAATGCATGGGTTGTGAGATTGCACANCA
GACCTGAAAAAATCTCATAAACACAAACAGATGTCCTGANAAATGTGCTACGAAACCCAC
TCTGTACTGCTGCTATGGCANTGCCATG
Restriction Sites NotI-NotI |
ACCN NM_004827
Insert Size 1968 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004827.2, NP_004818.2
RefSeq Size 4445 bp
RefSeq ORF 1968 bp
Locus ID 9429
UniProt ID Q9UNQ0
Cytogenetics 4q22.1
Domains AAA, ABC_tran
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways ABC transporters
Summary The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2012
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_004827" in other vectors (7)
SKU Description Size Price
RC205640 ABCG2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) 10 ug
$289.00 $914.00
RC205640L1 Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged 10 ug
$1,214.00
RC205640L2 Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged 10 ug
$1,214.00
RC205640L3 Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), Myc-DDK-tagged 10 ug
$1,214.00
RC205640L4 Lenti ORF clone of Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2), mGFP tagged 10 ug
$1,214.00
RG205640 ABCG2 (tGFP-tagged) - Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) 10 ug
$489.00 $1,114.00
SC320948 ABCG2 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 2 (ABCG2) 10 ug
$916.00

file-alt Citations

In vitro and in vivo identification of ABCB1 as an efflux transporter of bosutinib
Redaelli, Perini, Ceccon et al
J Hematol Oncol (2015) 8, 81 DOI: 10.1186/s13045-015-0179-4
Cited by 23
AI Summary: OriGene’s pCMV6-XL5 plasmid carrying the ABCG2 gene was used to generate K562BCRP cell lines, enabling the study of drug transporter roles in bosutinib resistance.
Snippet: UK)[ 12 ]. K562BCRP cell line was obtained by electroporation using a pcDNA3.1 plasmid carrying a ABCG2 gene-coding sequence isolated from the carrier plasmid pCMV6-XL5 obtained from Origene (Rockville, MD). Stable ... Cite SaveSaving
FigureFigureFigure
Endocytosis of ABCG2 drug transporter caused by binding of 5D3 antibody: trafficking mechanisms and intracellular fate
Studzian, Bartosz, Pulaski
Biochim. Biophys. Acta (2015)
Cited by 17
AI Summary: In this study, OriGene’s TrueClone cDNA for human ABCG2 was used as the template to construct a TagGFP2-ABCG2 fusion protein, enabling the investigation of ABCG2 endocytosis and trafficking mechanisms.
Snippet: plasmid for TagGFP2-ABCG2 fusion protein was constructed using pcDNA6.2/V5-DEST vector backbone (Life Technologies), coding sequence of human ABCG2 protein, amplified by high-fidelity PCR from commercial cDNA (TrueClone vector;Origene, ... Cite SaveSaving
Influence of HIV antiretrovirals on methadone N-demethylation and transport
Campbell, Gadel, Friedel et al
Biochem. Pharmacol. (2015)
Cited by 17
AI Summary: OriGene contributed to this study by providing the full-length human ABCG2 (BCRP) cDNA clone, which was used to construct BCRP-expressing cell lines for investigating drug transporter interactions with methadone metabolites.
Snippet: A BCRP (ABCG2)-expressing cell line was constructed in Madin-Darby Canine Kidney (MDCK) cells. The full-length cDNA clone of the human ABCG2 wild-type gene was acquired from Origene (Rockville, MD) and used without further modification. ... Cite SaveSaving
Involvement of Extracellular Vesicle long non-coding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
Takahashi, Yan, Wood et al
Mol. Cancer Res. (2014)
AI Summary: OriGene’s pCMV6-XL5-ABCG2 plasmid was used in this study to overexpress the ABCG2 protein in hepatocellular carcinoma cells, enabling the researchers to investigate the role of ABCG2 in modulating the effects of linc-VLDLR knockdown on chemotherapy-induced cell death.
Snippet: CA). Transfection of plasmids. pcDNA3.1(-)-MRP1 (ABCC-1) was kindly provided by Dr. Susan Cole at Queens University Cancer Research Institute, Kingston, Ontario, Canada. pCMV6-XL5-ABCG2 was purchased from OriGene Technologies (Rockville, MD). HepG2 ... Cite SaveSaving
Impact of genetic variability in the ABCG2 gene on ABCG2 expression, function, and interaction with AT1 receptor antagonist telmisartan
Deppe, Ripperger, Weiss et al
Biochem. Biophys. Res. Commun. (2014)
Cited by 30
AI Summary: OriGene’s pCMV6-XL5-ABCG2 plasmid was used as the source of human ABCG2 cDNA to generate expression constructs for studying the effects of ABCG2 genetic variants on protein expression and function in this paper.
Snippet: 2.1. Preparation of the pTRE-Tight-BI-AcGFP1–ABCG2 expression vector. The pCMV6-XL5 vector containing a 2700 bp fragment of the human ABCG2 mRNA sequence (pCMV6-XL5-ABCG2;NM_004827.2) was purchased from OriGene Technologies Inc. ... Cite SaveSaving
Étude de la perméabilité intestinale des médicaments par la reconstitution du transporteur BCRP/ABCG2 dans des protéoliposomes
Akik
Thesis (2018)
Snippet: Le clonage de BCRP a été réalisé en utilisant les produits suivants : plasmide donneur pCMV-XL5 (SC117127, OriGene), plasmide receveur pJ902-15 (ATUM), Platinum Pfx DNA polymerase (11708-013, Invitrogen), EcoRI-HF (R3101T, NEB), NotI-HF (R3189S, NEB), Anza T4 DNA Ligase Master Mix 4´ (IVGN2102, Invitrogen), agarose (800-015-CG, Wisent), RedSafe Nucleic Acid Staining Solution (21141, Intron Biotechnology), 1Kb DNA ladder RTU (DM010-R500, GeneDirex), QIAquick Gel Extraction Kit (28706, Qiagen), tryptone (BP1421- 100, Fisher), chlorure de sodium (793566, Sigma), extrait de levures (212750, Becton Dickinson), agar (214530, Becton Dickinson), zéocine (R250-01, Invitrogen), NEB-5a competent E. coli (C2987H, NEB), EZ-10 Spin Column Plasmid DNA Minipreps Kit (BS614, BioBasic Canada Inc), GenElute HP Endotoxin-Free Plasmid Maxiprep kit (NA0410, Sigma) et le rotor Sorvall SLA-1500 Super-Lite (Thermo Fisher Scientific)
Showing 1-6 of 6 papers.
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