TRPM4 (NM_017636) Human Tagged ORF Clone
- TrueORF®
SKU
RG216888
TRPM4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1
$789.00
$1,257.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TRPM4 |
| Synonyms | EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017636 |
| ORF Size | 3642 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017636.4 |
| RefSeq Size | 4061 bp |
| RefSeq ORF | 3645 bp |
| Locus ID | 54795 |
| UniProt ID | Q8TD43 |
| Cytogenetics | 19q13.33 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Summary | The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017636" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216888 | TRPM4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1 | 10 ug |
$1,057.00
|
|
| RC216888L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,357.00
|
|
| RC216888L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, mGFP tagged | 10 ug |
$1,357.00
|
|
| RC216888L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,357.00
|
|
| RC216888L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, mGFP tagged | 10 ug |
$1,357.00
|
|
| SC123991 | TRPM4 (untagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1 | 10 ug |
$1,058.00
|
|
Citations
Discovery of selective Orai channel blockers bearing an indazole or a pyrazole scaffold
Liardo, Pham, Ghilardi et al
Eur J Med Chem (2024) 278, 116805 DOI: 10.1016/j.ejmech.2024.116805
Liardo, Pham, Ghilardi et al
Eur J Med Chem (2024) 278, 116805 DOI: 10.1016/j.ejmech.2024.116805
Cited by 2
AI Summary: OriGene's GFP-tagged human TRPM4 plasmid was used to transfect HEK 293 cells, enabling the study of TRPM4 channel activity and the selectivity of the newly discovered CRAC channel blockers.
Snippet: GFP-tagged human TRPM4 plasmid (OriGene, Rockville, MD) was transfected into HEK 293 cells and recordings were made 1–2 days after transfection. The pipette solution contained 112 mM glutamic acid, 2 mM EGTA, 2 mM MgCl2, 1.5–1.7 mM CaCl2, pH 7.3 with CsOH. Endogenous TRPM7 channel current in Jurkat T cells was recorded as previously
Showing 1-1 of 1 papers.
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