TRPM4 (NM_017636) Human Tagged ORF Clone
SKU
RC216888
TRPM4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1
$1,057.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TRPM4 |
| Synonyms | EKVP6; hTRPM4; LTrpC4; PFHB1B; TRPM4B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017636 |
| ORF Size | 3642 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017636.4 |
| RefSeq Size | 4061 bp |
| RefSeq ORF | 3645 bp |
| Locus ID | 54795 |
| UniProt ID | Q8TD43 |
| Cytogenetics | 19q13.33 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 134.1 kDa |
| Summary | The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017636" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216888L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,357.00
|
|
| RC216888L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, mGFP tagged | 10 ug |
$1,357.00
|
|
| RC216888L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,357.00
|
|
| RC216888L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1, mGFP tagged | 10 ug |
$1,357.00
|
|
| RG216888 | TRPM4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1 | 10 ug |
$789.00
$1,257.00
|
|
| SC123991 | TRPM4 (untagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1 | 10 ug |
$1,058.00
|
|
Citations
Development and in vitro characterization of humanized antibodies for blocking human TRPM4 channel
Wei, Poore, Verma et al
Sci Rep (2025) 15 (1), 19769 DOI: 10.1038/s41598-025-05256-x
Wei, Poore, Verma et al
Sci Rep (2025) 15 (1), 19769 DOI: 10.1038/s41598-025-05256-x
Cited by 2
AI Summary: OriGene’s myc-tagged human TRPM4 plasmid (#RC216888) was used to transfect HEK 293 cells, enabling the evaluation of antibody binding, surface labeling, and functional inhibition of the human TRPM4 channel in this study.
Snippet: antibody binding to peptide-biotin was individually determined using the Biacore 8 K. Surface labelling and immunocytochemistry HEK 293 cells were transfected with myc-tagged human TRPM4 (#RC216888, OriGene Technologies, Inc.) using lipofectamine 2000 transfection reagent (#11668019, Thermo Fisher Scientific). One day after transfection, the cells were incubated with IgG, M4P and M4M at a concent
Acetalax (Oxyphenisatin acetate, NSC 59687) and bisacodyl Cause Oncosis in Triple Negative Breast Cancer Cell lines by Poisoning Ion Exchange Membrane Protein TRPM4
Mizunuma, Redon, Saha et al
Cancer Res Commun (2024)
Mizunuma, Redon, Saha et al
Cancer Res Commun (2024)
Showing 1-2 of 2 papers.
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