P Glycoprotein (ABCB1) (NM_000927) Human Tagged ORF Clone
- TrueORF®
SKU
RG216080
ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1)
$789.00
$1,871.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | P Glycoprotein |
| Synonyms | ABC20; CD243; CLCS; GP170; MDR1; p-170; P-GP; PGY1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000927 |
| ORF Size | 3840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000927.3, NP_000918.2 |
| RefSeq Size | 4872 bp |
| RefSeq ORF | 3843 bp |
| Locus ID | 5243 |
| UniProt ID | P08183, A4D1D2 |
| Cytogenetics | 7q21.12 |
| Domains | AAA, ABC_membrane, ABC_tran |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | ABC transporters |
| Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. provided by RefSeq, Feb 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000927" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216080 | ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$589.00
$1,671.00
|
|
| RC216080L1 | Lenti-ORF clone of ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L2 | Lenti-ORF clone of ABCB1 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L3 | Lenti-ORF clone of ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L4 | Lenti-ORF clone of ABCB1 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| SC119584 | ABCB1 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,672.00
|
|
Citations
Characterization of P-glycoprotein orthologs from human, sheep, pig, dog, and cat
Azimi, Yee, Riselli et al
J Vet Pharmacol Ther (2023)
Azimi, Yee, Riselli et al
J Vet Pharmacol Ther (2023)
Cited by 9
AI Summary: OriGene products, specifically the empty vector (EV, catalog #PS100010) and human ABCB1 plasmid (catalog #RG216080) in the pCMV6-AC-GFP backbone, were used to generate control and human P-glycoprotein-expressing HEK293 cells for functional comparison with other species' P-gp orthologs in this study.
Snippet: Empty vector (EV) (catalog #PS100010) and human ABCB1 (catalog #RG216080;NM_000927) encoding plasmids in the pCMV6-AC-GFP vector backbone were purchased from Origene Technologies. Full-length cDNAs for sheep (AM491812.1), pig (KP233220.1), dog (NM_001003215.2), and cat (NM_001171064.2) ABCB1 genes were synthesized and cloned into the pcDNA3.1 + C-eGFP vector backbone by GenScript, Inc. …
Leveraging Mathematical Modeling to Quantify Pharmacokinetic and Pharmacodynamic Pathways: Equivalent Dose Metric
McKenna, Weis, Quaranta et al
Front Physiol (2019) 10, 616 DOI: 10.3389/fphys.2019.00616
McKenna, Weis, Quaranta et al
Front Physiol (2019) 10, 616 DOI: 10.3389/fphys.2019.00616
Cited by 9
AI Summary: OriGene products, specifically the ABCB1 (MDR1) expression plasmid (Cat# RC211445) and ABCB1 shRNA plasmids (Cat# TL313374), were used to modulate and study the expression of drug efflux pumps in cell lines, enabling the authors to assess the impact of drug efflux on doxorubicin pharmacokinetics and pharmacodynamics in their mathematical modeling framework.
Doxorubicin-conjugated dexamethasone induced MCF-7 apoptosis without entering the nucleus and able to overcome MDR-1-induced resistance
Chaikomon, Chattong, Chaiya et al
Drug Des Devel Ther (2018) 12, 2361-2369 DOI: 10.2147/DDDT.S168588
Chaikomon, Chattong, Chaiya et al
Drug Des Devel Ther (2018) 12, 2361-2369 DOI: 10.2147/DDDT.S168588
AI Summary: OriGene's ABCB1 pCMV GFP-tagged plasmid was used to transfect MCF-7 cells and induce drug resistance, enabling the researchers to evaluate the effectiveness of the new doxorubicin-dexamethasone conjugate against multidrug-resistant cancer cells.
Snippet: MDR-1 overexpression. To stimulate drug-resistance in MCF-7, we transfected MCF-7 cells with an ABCB1 pCMV GFP-tagged plasmid (OriGene Technologies Inc., Rockville, MD, USA). One day prior to transfection, the cells were plated at 10,000 cells per cm 2 in a
Variable Cell Line Pharmacokinetics Contribute to Non-Linear Treatment Response in Heterogeneous Cell Populations
McKenna, Weis, Quaranta et al
Ann Biomed Eng (2018)
McKenna, Weis, Quaranta et al
Ann Biomed Eng (2018)
AI Summary: OriGene’s GFP-tagged MDR1 (ABCB1 gene) product was used to generate MDR1-overexpressing breast cancer cell lines, enabling the study of how drug efflux impacts treatment response in heterogeneous cell populations.
Snippet: a monomeric red fluorescence protein (mRFP)-tagged H2B protein as described previously.12 The H2BmRFP-expressing MDA-MB-468 cell line was again transduced to ex- press green fluorescent protein (GFP)-tagged MDR1 (ABCB1 gene, Origene Technologies
Saikosaponins A, C and D enhance liver-targeting effects of anticancer drugs by modulating drug transporters
Feng, Liu, Zhao et al
Oncotarget (2017) 8 (66), 110092-110102 DOI: 10.18632/oncotarget.22639
Feng, Liu, Zhao et al
Oncotarget (2017) 8 (66), 110092-110102 DOI: 10.18632/oncotarget.22639
Snippet: Human Pgp and GAPDH primers were synthesized by Invitrogen (Guangzhou, China). OCT2-pCMV6-AC-GFP, Mrp2-pCMV6-AC-GFP, ABCB1-pCMV6-AC-GFP, and pCMV6-AC-GFP plasmids and Mega Tran1.0 transfection reagent were purchased from OriGene Technologies Company (Maryland, USA). …
Clerosterol from vinegar-baked radix bupleuri modifies drug transport
Zhao, Feng, Liu et al
Oncotarget (2017) 8 (13), 21351-21361 DOI: 10.18632/oncotarget.15212
Zhao, Feng, Liu et al
Oncotarget (2017) 8 (13), 21351-21361 DOI: 10.18632/oncotarget.15212
Snippet: MA). OCT2-pCMV6-AC-GFP, Mrp2-pCMV6-AC-GFP, ABCB1-pCMV6-AC- GFP, and pCMV6-AC-GFP plasmids and Mega Tran 1.0 transfection reagent were purchased from OriGene Technologies Company (USA). Aminoglycoside ... All 3 versions Cite SaveSaving
Showing 1-6 of 11 papers.
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