P Glycoprotein (ABCB1) (NM_000927) Human Tagged ORF Clone
- TrueORF®
SKU
RC216080
ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1)
$589.00
$1,671.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | P Glycoprotein |
| Synonyms | ABC20; CD243; CLCS; GP170; MDR1; p-170; P-GP; PGY1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000927 |
| ORF Size | 3840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000927.4 |
| RefSeq Size | 4872 bp |
| RefSeq ORF | 3843 bp |
| Locus ID | 5243 |
| UniProt ID | P08183, A4D1D2 |
| Cytogenetics | 7q21.12 |
| Domains | AAA, ABC_membrane, ABC_tran |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | ABC transporters |
| MW | 141.3 kDa |
| Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. provided by RefSeq, Feb 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000927" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216080L1 | Lenti-ORF clone of ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L2 | Lenti-ORF clone of ABCB1 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L3 | Lenti-ORF clone of ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RC216080L4 | Lenti-ORF clone of ABCB1 (mGFP-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,971.00
|
|
| RG216080 | ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$789.00
$1,871.00
|
|
| SC119584 | ABCB1 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) | 10 ug |
$1,672.00
|
|
Citations
Design and Synthesis of Cyclolipopeptide Mimics of Dysoxylactam A and Evaluation of the Reversing Potencies against P-Glycoprotein-Mediated Multidrug Resistance
Yang, Wang, Gao et al
J Med Chem (2024) 67 (6), 4560-4582 DOI: 10.1021/acs.jmedchem.3c01920
Yang, Wang, Gao et al
J Med Chem (2024) 67 (6), 4560-4582 DOI: 10.1021/acs.jmedchem.3c01920
Cited by 14
AI Summary: OriGene products contributed to this study by providing the ABCB1 (OriGene RC216080) and ABCG2 (OriGene RC205640L3) plasmids, which were used to generate drug-resistant HEK293 cell lines for evaluating the ability of synthesized cyclolipopeptides to reverse P-glycoprotein-mediated multidrug resistance.
Snippet: Sciences.HEK293/ABCB1,HEK293/ABCG2,and HEK293/ABCC1cellsweregeneratedby transfectingHEK293cellswithABCB1(OrigeneRC216080),ABCG2(OrigeneRC205640L3),and ABCC1(YouBioG152637)plasmidsaccordingto the instructions,respectively,and werecollectedfor the followi
Temozolomide reverses doxorubicin resistance by inhibiting P-glycoprotein in malignant glioma cells
Zhang, Saito, Shibahara et al
J. Neurooncol. (2015)
Zhang, Saito, Shibahara et al
J. Neurooncol. (2015)
Cited by 33
MDR1 Transporter Protects Against Paraquat-Induced Toxicity in Human and Mouse Proximal Tubule Cells
Wen, Gibson, Yang et al
Toxicol. Sci. (2014)
Wen, Gibson, Yang et al
Toxicol. Sci. (2014)
Cited by 33
AI Summary: OriGene’s pCMV6-NEO plasmid containing the full-length human MDR1 gene was used to stably transfect HEK293 cells, enabling the study of MDR1’s role in paraquat transport and toxicity.
Snippet: Human MDR1 transfection. HEK293 cells were transfected with the pCMV6-NEO plasmid containing no insert (empty vector, EV) or the full-length human MDR1 gene (Origene, Rockville, MD) using lipofectamine LTX and PLUS reagents (Invitrogen, Carlsbad, CA). ... Cite SaveSaving
Doxorubicin and siRNA Co-Delivery via Chitosan-Coated pH-Responsive Mixed Micellar Polyplexes for Enhanced Cancer Therapy in Multidrug-Resistant Tumors
Butt, Amin, Katas et al
Molecular Pharmaceutics (2016)
Butt, Amin, Katas et al
Molecular Pharmaceutics (2016)
AI Summary: OriGene contributed to the study by providing plasmid vectors with ORF clones of human ABC, sub-family B, member 1 (MDR1/ABCB1), which were used to investigate drug resistance mechanisms in cancer therapy.
Snippet: Plasmid vector with ORF clones of human ABC, sub-family B, member 1(MDR1/ABCB1) were obtained from Origene (Rockville, MD, USA). Lipofectamine RNAi Maxtransfection reagent, trypsin-ethylenediaminetetraacetic acid (EDTA), Roswell Park Memorial ... Cite SaveSaving
Showing 1-4 of 4 papers.
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