FLT3 (NM_004119) Human Tagged ORF Clone
$1,563.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FLT3 |
| Synonyms | CD135; FLK-2; FLK2; STK1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004119 |
| ORF Size | 2979 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004119.1, NP_004110.1 |
| RefSeq Size | 3475 bp |
| RefSeq ORF | 2982 bp |
| Locus ID | 2322 |
| UniProt ID | P36888 |
| Cytogenetics | 13q12.2 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane |
| Protein Pathways | Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer |
| Summary | This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. provided by RefSeq, Jan 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004119" in other vectors (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211459 | FLT3 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 3 (FLT3) | 10 ug |
$1,363.00
|
|
| RC211459L1 | Lenti ORF clone of Human fms-related tyrosine kinase 3 (FLT3), Myc-DDK-tagged | 10 ug |
$1,663.00
|
|
| RC211459L2 | Lenti ORF clone of Human fms-related tyrosine kinase 3 (FLT3), mGFP tagged | 10 ug |
$1,663.00
|
|
| RC211459L3 | Lenti ORF clone of Human fms-related tyrosine kinase 3 (FLT3), Myc-DDK-tagged | 10 ug |
$1,663.00
|
|
| RC211459L4 | Lenti ORF clone of Human fms-related tyrosine kinase 3 (FLT3), mGFP tagged | 10 ug |
$1,663.00
|
|
| RC600013 | FLT3 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens fms-related tyrosine kinase 3, Signal peptide (1-26) plus EC domain (27-543) | 10 ug |
$289.00
$505.00
|
|
| SC124086 | FLT3 (untagged)-Human fms-related tyrosine kinase 3 (FLT3) | 10 ug |
$1,365.00
|
|
| SC323509 | FLT3 (untagged)-Kinase deficient mutant (K644M) of Human fms-related tyrosine kinase 3 (FLT3) | 10 ug |
$1,365.00
|
|
Citations
Human Cytomegalovirus Encodes a Novel FLT3 Receptor Ligand Necessary for Hematopoietic Cell Differentiation and Viral Reactivation
Crawford, Kim, Collins-McMillen et al
MBio (2018) 9 (2) DOI: 10.1128/mBio.00682-18
Crawford, Kim, Collins-McMillen et al
MBio (2018) 9 (2) DOI: 10.1128/mBio.00682-18
AI Summary: OriGene’s FLT3 (GFP-tagged)-human fms-tyrosine kinase 3 plasmid (catalog no. RG211459) was used to generate stable HEK-293 cells expressing the Flt-3 receptor, enabling the study of UL7’s interaction with Flt-3R in hematopoietic cell differentiation and HCMV reactivation.
Snippet: Stable transfected HEK-293 cells expressing human Flt-3 receptor (Flt-3R) (HEK-293-hFlt-3R) were generated by transfection of FLT3 (green fluorescent protein [GFP]-tagged)-human fms-tyrosine kinase 3 plasmid (catalog no. RG211459;OriGene Technologies) with Lipofectamine 2000 (Life Technologies) followed by G418 selection (800 g/ml)
Showing 1-1 of 1 papers.
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